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Entrapment of Pseudomonas cepacia lipase with peracetylated β-cyclodextrin in sol-gel: application to the kinetic resolution of secondary alcohols
journal contribution
posted on 2023-05-17, 00:00 authored by Ghanem, A, Schurig, VCo-lyophilized Pseudomonas cepacia lipase with peracetylated β-cyclodextrin was immobilized by the sol–gel process. The gel-entrapped lipase/cyclodextrin was prepared by the hydrolysis of methyltrimethoxysilane (MTMS) in the presence of the co-lyophilized lipase with peracetylated β-cyclodextrin prepared with different weight ratios (enzyme to CD). This type of enzyme preparation was subsequently used in the kinetic resolution of a set of secondary alcohols using isopropenyl acetate as an innocuous acyl donor in toluene as the organic medium. The resulting chiral alcohols (substrate) and the corresponding acetates (product) were baseline separated in one analysis without derivatization using gas chromatography on a new chiral stationary phase (CSP) Chirasil-β-Dex containing an undecamethylene spacer (C11-Chirasil-Dex).
History
Publication title
Tetrahedron: AsymmetryVolume
14Issue
17Pagination
2547-2555ISSN
0957-4166Department/School
School of Natural SciencesPublisher
ElsevierPlace of publication
AmsterdamRights statement
The definitive version is available at http://www.sciencedirect.comRepository Status
- Restricted