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Entrapment of Pseudomonas cepacia lipase with peracetylated β-cyclodextrin in sol-gel: application to the kinetic resolution of secondary alcohols
Citation
Ghanem, A and Schurig, V, Entrapment of Pseudomonas cepacia lipase with peracetylated β-cyclodextrin in sol-gel: application to the kinetic resolution of secondary alcohols , Tetrahedron: Asymmetry, 14, (17) pp. 2547-2555. ISSN 0957-4166 (2003) [Refereed Article]
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DOI: doi:10.1016/S0957-4166(03)00550-0
Abstract
Co-lyophilized Pseudomonas cepacia lipase with peracetylated β-cyclodextrin was immobilized by the sol–gel process. The gel-entrapped lipase/cyclodextrin was prepared by the hydrolysis of methyltrimethoxysilane (MTMS) in the presence of the co-lyophilized lipase with peracetylated β-cyclodextrin prepared with different weight ratios (enzyme to CD). This type of enzyme preparation was subsequently used in the kinetic resolution of a set of secondary alcohols using isopropenyl acetate as an innocuous acyl donor in toluene as the organic medium. The resulting chiral alcohols (substrate) and the corresponding acetates (product) were baseline separated in one analysis without derivatization using gas chromatography on a new chiral stationary phase (CSP) Chirasil-β-Dex containing an undecamethylene spacer (C11-Chirasil-Dex).
Item Details
Item Type: | Refereed Article |
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Keywords: | efficient heterogeneous biocatalysts, organically modified silicates, enhanced reaction rate, immobilized lipase, transesterification, esterification, hydrolysis, solvents, enzymes,, matrix |
Research Division: | Chemical Sciences |
Research Group: | Organic chemistry |
Research Field: | Organic green chemistry |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Ghanem, A (Dr Ashraf Ghanem) |
ID Code: | 57754 |
Year Published: | 2003 |
Web of Science® Times Cited: | 25 |
Deposited By: | Austn Centre for Research in Separation Science |
Deposited On: | 2009-08-12 |
Last Modified: | 2013-01-22 |
Downloads: | 0 |
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