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On-column titration and investigation of metal complex formation for aminopolycarboxylate functionalised monoliths using scanning contactless conductivity detection

Citation

Gillespie, E and Connolly, D and Nesterenko, PN and Paull, B, On-column titration and investigation of metal complex formation for aminopolycarboxylate functionalised monoliths using scanning contactless conductivity detection, Journal of Separation Science, 32, (15-16) pp. 2659-2667. ISSN 1615-9306 (2009) [Refereed Article]


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The definitive published version is available online at: http://interscience.wiley.com

Official URL: http://interscience.wiley.com

DOI: doi:10.1002/jssc.200900232

Abstract

The application of scanning capacitively coupled contactless conductivity detection (SC4D) for the determination of pH dependant behaviour of two aminopolycarboxylates immobilised onto the surface of a monolithic capillary column is described. The use of SC4D to visualise changes in conductivity of the discrete zones of functional groups within monolithic capillary columns allows for the effects of immobilisation on the physicochemical properties of zones to be compared to that of the functional group in solution. The perturbation of the pKa values of the functional groups can be attributed to the change in chemical environment experienced by the functional group through the presence of local hydrogen bonding and surface induced effects. These bonds, both between adjacent functional groups and with the monolithic polymethacrylate scaffold, result in a modification of the electron density on the functional group and therefore a change in pKa. Changes in the pKa of N-(2-acetamido)iminodiacetic acid (ADA) from 2.48 to 5.2 for one of the acidic protons, with little change in the pKa of the amine group, were observed, which correlates to similar changes in aggregated systems of aminopolycarboxylates. Similar results were obtained for the iminodiacetic acid (IDA) once immobilised onto the surface of the monolith. Furthermore, the ability to measure changes in the charge of such discrete zones of functional groups allows for the visualisation of complexation events occurring directly on-column, where such complexes result in a change in charge of the functional group. This potentially useful technique is illustrated within for the formation of aminopolycarboxylate complexes with a selection of metal ions.

Item Details

Item Type:Refereed Article
Keywords:Complexation � Contactless conductivity � Monolith � Photografting � Titration
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Nesterenko, PN (Professor Pavel Nesterenko)
Author:Paull, B (Professor Brett Paull)
ID Code:57724
Year Published:2009
Web of Science® Times Cited:14
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2009-08-10
Last Modified:2014-11-21
Downloads:1 View Download Statistics

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