Gibberellin A4 (GA4) was identified for the first time in the garden pea (Pisum sativum) L.), by gas chromatography-mass spectrometry. However, in wild-type shoots the level of GA4 was only about 6% of the level of GA1, and it is therefore unlikely that GA4 plays a major role per se in the control of pea stem elongation. In shoots of the le mutant, GA4 was not detected, while the level of GA9 was approximately twice that found in the wild-type. The le mutation also markedly reduced the elongation response to applied GA9. It appears, therefore, that in Pisum the le mutation blocks the 3β-hydroxylation of GA9 to GA4, in addition to the 3β-hydroxylation of GA20 to GA1. In contrast, the le mutation did not reduce the response to applied GA5, suggesting the step GA5 to GA3 is not catalysed by the enzyme controlled by the Le gene. The step GA5 to GA3 was confirmed in peas by metabolite analysis after treatment with deuterated GA5. © 1995 Kluwer Academic Publishers.

Item Type:	Refereed Article
Research Division:	Biological Sciences
Research Group:	Plant biology
Research Field:	Plant physiology
Objective Division:	Expanding Knowledge
Objective Group:	Expanding knowledge
Objective Field:	Expanding knowledge in the environmental sciences
UTAS Author:	Poole, AT (Mr Andrew Thomas Poole)
UTAS Author:	Ross, JJ (Associate Professor John Ross)
UTAS Author:	Lawrence, NL (Ms Naomi Leigh Lawrence)
UTAS Author:	Reid, JB (Professor Jim Reid)
ID Code:	5668
Year Published:	1995
Web of Science® Times Cited:	11
Deposited By:	Plant Science
Deposited On:	1995-08-01
Last Modified:	2010-11-08
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