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Use of a biomimetic chromatographic stationary phase for study of the interactions occurring between inorganic anions and phosphatidylcholine membranes

Citation

Hu, W and Haddad, PR and Hasebe, K and Mori, M and Tanaka, K and Ohno, M and Kamo, N, Use of a biomimetic chromatographic stationary phase for study of the interactions occurring between inorganic anions and phosphatidylcholine membranes, Biophysical Journal, 83, (6) pp. 3351-3356. ISSN 0006-3495 (2002) [Refereed Article]


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Copyright Statement

Copyright © 2002 The Biophysical Society Published by Elsevier Inc. The definitive version is available at http://www.sciencedirect.com

Official URL: http://www.elsevier.com

DOI: doi:10.1016/S0006-3495(02)75335-6

Abstract

A liquid chromatographic method for the study of ion-membrane interactions is reported. A phosphatidylcholine biomimetic stationary phase was established by loading dimyristoylphosphatidylcholine (DMPC) onto a reversed-phase octadecylsilica packed column. This column was then used to study the interaction of some inorganic anions with the stationary phase by UV and conductivity detection. Ten inorganic anions were selected as model ions and were analyzed with the proposed chromatographic system. Anion-DMPC interactions of differing magnitudes were observed for all of the model anions. Perchlorate-DMPC interactions were strongest, followed by thiocyanate-DMPC, iodide-DMPC, chlorate-DMPC, nitrate-DMPC, bromide-DMPC, chloride-DMPC, fluoride-DMPC, and then sulfate-DMPC. Cations in the eluent, especially H+ ions and divalent cations such as Ca2+, showed strong effects on anion-DMPC interactions. The chromatographic data suggest that DMPC interacts with both the anions and the cations. Anion-DMPC interactions were dependent on the surface potential of the stationary phase: at low surface potentials anion-DMPC interactions were predominantly solvation dependent in nature whereas at more positive surface potentials anion-DMPC interactions were predominantly electrostatic in nature. Cation-DMPC interactions served to raise the surface potential, causing the anion-DMPC interactions to vary from solvation dependent to electrostatic. The chromatographic data were used to provide quantitative estimates of the enthalpies of the anion-DMPC interactions.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Haddad, PR (Professor Paul Haddad)
Author:Tanaka, K (Dr Kazuhiko Tanaka)
ID Code:56452
Year Published:2002
Web of Science® Times Cited:9
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2009-05-04
Last Modified:2009-07-28
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