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Separation of transition metals on a poly-iminodiacetic acid grafted polymeric resin column with post-column reaction detection utilising a paired emitter-detector diode system

Citation

Barron, L and O'Toole, M and Diamond, D and Nesterenko, PN and Paull, B, Separation of transition metals on a poly-iminodiacetic acid grafted polymeric resin column with post-column reaction detection utilising a paired emitter-detector diode system, Journal of Chromatography A, 1213, (1) pp. 31-36. ISSN 0021-9673 (2008) [Refereed Article]

DOI: doi:10.1016/j.chroma.2008.08.049

Abstract

The selectivity, retention and separation of transition metals on a short (2 mm × 50 mm) column packed with a poly-iminodiacetic acid functionalised polymer 10 μm resin (Dionex ProPac IMAC-10®) are presented. This stationary phase, typically used for the separation of proteins, is composed of long chain poly-iminodiacetic acid groups grafted to a hydrophilic layer surrounding a 10 μm polymeric bead. Through the use of a combination of a multi-step pH and picolinic acid gradient, the separation of magnesium, iron, cobalt, cadmium, zinc, lead and copper was possible, followed by post-column reaction with 4-(2-pyridylazo) resorcinol (PAR) and absorbance detection at 510 nm using a novel and inexpensive optical detector, comprised of two light emitting diodes with one acting as a light source and the other as a detector. Column efficiency for selective transition metals was in excess of N = 10,000, with the baseline separation of seven metal cations in <3 min possible under optimised conditions. Detection limits of between 5 and 81 μg/L were possible based upon a 50 μL injection volume. © 2008 Elsevier B.V. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Nesterenko, PN (Professor Pavel Nesterenko)
Author:Paull, B (Professor Brett Paull)
ID Code:55258
Year Published:2008
Web of Science® Times Cited:27
Deposited By:Chemistry
Deposited On:2009-03-06
Last Modified:2015-01-20
Downloads:0

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