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Separation of transition metals on a poly-iminodiacetic acid grafted polymeric resin column with post-column reaction detection utilising a paired emitter-detector diode system
Citation
Barron, L and O'Toole, M and Diamond, D and Nesterenko, PN and Paull, B, Separation of transition metals on a poly-iminodiacetic acid grafted polymeric resin column with post-column reaction detection utilising a paired emitter-detector diode system, Journal of Chromatography A, 1213, (1) pp. 31-36. ISSN 0021-9673 (2008) [Refereed Article]
DOI: doi:10.1016/j.chroma.2008.08.049
Abstract
The selectivity, retention and separation of transition metals on a short (2 mm × 50 mm) column packed with a poly-iminodiacetic acid functionalised polymer 10 μm resin (Dionex ProPac IMAC-10®) are presented. This stationary phase, typically used for the separation of proteins, is composed of long chain poly-iminodiacetic acid groups grafted to a hydrophilic layer surrounding a 10 μm polymeric bead. Through the use of a combination of a multi-step pH and picolinic acid gradient, the separation of magnesium, iron, cobalt, cadmium, zinc, lead and copper was possible, followed by post-column reaction with 4-(2-pyridylazo) resorcinol (PAR) and absorbance detection at 510 nm using a novel and inexpensive optical detector, comprised of two light emitting diodes with one acting as a light source and the other as a detector. Column efficiency for selective transition metals was in excess of N = 10,000, with the baseline separation of seven metal cations in <3 min possible under optimised conditions. Detection limits of between 5 and 81 μg/L were possible based upon a 50 μL injection volume. © 2008 Elsevier B.V. All rights reserved.
Item Details
Item Type: | Refereed Article |
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Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Nesterenko, PN (Professor Pavel Nesterenko) |
UTAS Author: | Paull, B (Professor Brett Paull) |
ID Code: | 55258 |
Year Published: | 2008 |
Web of Science® Times Cited: | 30 |
Deposited By: | Chemistry |
Deposited On: | 2009-03-06 |
Last Modified: | 2015-01-20 |
Downloads: | 0 |
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