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Packing procedures for high efficiency, short ion-exchange columns for rapid separation of inorganic anions


Tyrrell, EC and Hilder, EF and Shalliker, RA and Dicinoski, GW and Shellie, RA and Breadmore, MC and Pohl, CA and Haddad, PR, Packing procedures for high efficiency, short ion-exchange columns for rapid separation of inorganic anions, Journal of Chromatography A, 1208, (1-2) pp. 95-100. ISSN 0021-9673 (2008) [Refereed Article]

DOI: doi:10.1016/j.chroma.2008.08.056


An optimised packing procedure for the production of high efficiency, short, particle-packed ion-exchange columns is reported. Slurry-packing techniques were applied to a series of interconnected short columns, with the columns situated intermediate between the inlet and outlet ends of the series being used for separations. The fast separation and determination of inorganic anions was achieved using short (4 mm ID, 30 mm long) columns packed with Dionex AS20 high-capacity anion-exchange stationary phase. Seven inorganic anions (bromate, chloride, chlorate, nitrate, sulfate, chromate and perchlorate) are separated in 2.6 min using a hydroxide gradient and a flow-rate of 1.8 mL/min (total analysis time including re-equilibration was 3.5 min). Under isocratic conditions, the home-packed columns exhibited efficiency values of 43,000 N/m for chloride at a flow-rate of 0.3 mL/min, compared to 54,000 N/m for a commercial 250 mm AS20 column at the same flow-rate. However, the short columns gave approximately a threefold higher sample throughput. The short, home-packed columns could be produced reproducibly and gave consistent performance over extended periods of usage. Crown Copyright © 2008.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Tyrrell, EC (Dr Eadaoin Tyrrell)
UTAS Author:Hilder, EF (Professor Emily Hilder)
UTAS Author:Dicinoski, GW (Associate Professor Gregory Dicinoski)
UTAS Author:Shellie, RA (Associate Professor Robert Shellie)
UTAS Author:Breadmore, MC (Professor Michael Breadmore)
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:55254
Year Published:2008
Web of Science® Times Cited:18
Deposited By:Chemistry
Deposited On:2009-03-06
Last Modified:2009-05-01

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