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Anion-exchange chromatography on short reversed-phase columns modified with amphoteric (N-dodecyl-N,N-dimethylammonio)alcanoates


Nesterenko, EP and Nesterenko, PN and Paull, B, Anion-exchange chromatography on short reversed-phase columns modified with amphoteric (N-dodecyl-N,N-dimethylammonio)alcanoates, Journal of Chromatography A, 1178, (1-2) pp. 60-70. ISSN 0021-9673 (2008) [Refereed Article]

DOI: doi:10.1016/j.chroma.2007.11.038


Short reversed-phase columns (50 mm × 4.6 mm Gemini C18) were dynamically coated with carboxybetaines of the general structure, C12H25N+(CH3)2(CH2)nCOOH, namely (N-dodecyl-N,N-dimethylammonio)undecanoate, DDMAU (n = 10) and (N-dodecyl-N,N-dimethylammonio)butyrate, DDMAB (n = 3), and investigated for the separation of inorganic anions in ion chromatography. The role of the ionic strength of coating surfactant solutions on their adsorption and resultant column capacity was studied. The retention of inorganic anions was investigated with different eluents at various concentrations and pH. Interestingly, no retention for anions was found with pure water as the eluent, but the addition of small amounts of electrolytes, up to 0.1 mM, caused a sharp increase in the retention of analytes. The effect of increasing anion retention with an increase in eluent cation charge was also observed. Based on this effect a new cation charge gradient concept was proposed and applied to the separation of a standard mixture of anions. © 2007 Elsevier B.V. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Nesterenko, PN (Professor Pavel Nesterenko)
UTAS Author:Paull, B (Professor Brett Paull)
ID Code:55181
Year Published:2008
Web of Science® Times Cited:9
Deposited By:Chemistry
Deposited On:2009-03-06
Last Modified:2011-08-18

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