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Microwave and ultrasonic extraction of chlorophenoxy acids from soil and their determination by fluorescence polarization immunoassay

Citation

Morozova, VS and Eremin, SA and Nesterenko, PN and Klyuev, NA and Shelpchikov, AA and Kubrakova, IV, Microwave and ultrasonic extraction of chlorophenoxy acids from soil and their determination by fluorescence polarization immunoassay, Journal of Analytical Chemistry, 63, (2) pp. 127-134. ISSN 1061-9348 (2008) [Refereed Article]

DOI: doi:10.1134/S1061934808020044

Abstract

Procedures were developed for the ultrasonic and microwave extraction of pesticides, 2,4-dichlorophenoxyacetic (2,4-D) and 2,4,5-trichlophenoxyacetic (2,4,5-T) acids from soils for the subsequent determination by fluorescence polarization immunoassay (FPIA). The effect of the matrix composition of soils on the FPIA results was studied, and the optimum extractants and extraction conditions were selected. It was found that 40% ethanol is optimum for both extraction and FPIA determination, because it does not cause antibody denaturation. The recovery of pesticides in soil was 80-132% for 2,4-D and 101-138% for 2,4,5-T. Microwave extraction is more efficient than ultrasonic extraction for the determination of 2,4-D and 2,4,5-T in soil. The detection limit in soil and the analytical range are 2 and 4-200 μg/g, respectively, for 2,4-D and 20 and 80-5000 μg/g, respectively, for 2,4,5-T. Results of the determination of 2,4-D in soil by FPIA are in good agreement with the results of the determination by high-performance liquid chromatography. The procedures can be used for the rapid determination of chlorophenoxy acids in soils. © Pleiades Publishing, Ltd., 2008.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Nesterenko, PN (Professor Pavel Nesterenko)
ID Code:55179
Year Published:2008
Web of Science® Times Cited:8
Deposited By:Chemistry
Deposited On:2009-03-06
Last Modified:2009-05-01
Downloads:0

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