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Separation of inorganic anions on a high capacity porous polymeric monolithic column and application to direct determination of anions in seawater

journal contribution
posted on 2023-05-16, 23:03 authored by Evenhuis, CJ, Buchberger, WW, Emily HilderEmily Hilder, Flook, KJ, Pohl, CA, Nesterenko, PN, Paul HaddadPaul Haddad
A commercially available 4.6 mm. id × 50 mm polymethacrylate-based monolithic strong anion exchange column (ProSwift™ SAX-1S) designed for the separation of proteins has been successfully used to separate small inorganic anions in the presence of a seawater sample matrix. Using a hydroxide eluent with suppressed conductivity detection the ion exchange capacity of this column declined over time; however, using KC1 as the eluent, the column performance was stable with a capacity of 530 μequiv. for nitrate. The optimum conditions for the separation of iodate, bromate, nitrite, bromide and nitrate were assessed by constructing van Deemter plots using 1.00 and 0.100 M KC1. Efficiencies of up to 26 700 plates/m were recorded using 1.00 M KCl, at a flow rate of 0.20 mL/min but iodate was not baseline resolved from the void peak. By reducing the concentration of the eluent to 0.100 M, efficiencies of up to 39900 plates/m could be obtained at 0.35 mL/min. By employing a linear gradient ranging from 0.05 to 1.00 M KCl the ions dissolved in distilled water or a salt water matrix could be baseline separated in less than 3 min at a flow rate of 2.50 mL/min. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

History

Publication title

Journal of Separation Science

Volume

31

Issue

14

Pagination

2598-2604

ISSN

1615-9306

Department/School

School of Natural Sciences

Publisher

Wiley VCH

Place of publication

Weinheim

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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