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Brassinosteroids, de-etiolation and the re-emerging art of plant hormone quantification

Citation

Symons, GM and Reid, JB, Brassinosteroids, de-etiolation and the re-emerging art of plant hormone quantification, Plant Signaling & Behavior, 3, (10) pp. 868-70. ISSN 1559-2324 (2008) [Refereed Article]

DOI: doi:10.1007/s00425-007-0685-x

Abstract

An increase in the use of molecular techniques has provided a significant insight into the function of genes, and how they are regulated and interact. However, in the field of plant hormone physiology, the increased use of these techniques has been accompanied by a reduction in the direct measurement of plant hormone levels by physiochemical methods. Instead, the transcript (mRNA) levels of genes involved in hormone metabolism are often used to predict endogenous hormone levels. The validity of this approach was recently tested by comparing the expression of a range of genes involved in BR synthesis, catabolism and perception, with the actual endogenous BR levels in pea seedlings grown under different light conditions.1,2 Based on this comparison, we now argue that gene expression analysis alone is not always a reliable indicator of endogenous hormone levels.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural Crops
Objective Field:Horticultural Crops not elsewhere classified
Author:Symons, GM (Mr Gregory Symons)
Author:Reid, JB (Professor Jim Reid)
ID Code:55090
Year Published:2008
Deposited By:Plant Science
Deposited On:2009-03-05
Last Modified:2015-02-04
Downloads:0

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