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Capillary electrophoresis separation and native laser-induced fluorescence detection of metallotexaphyrins
Citation
Quirino, JP and Dulay, MT and Fu, L and Mody, TD and Zare, RN, Capillary electrophoresis separation and native laser-induced fluorescence detection of metallotexaphyrins, Journal of Separation Science, 25, (13) pp. 819-824. ISSN 1615-9314 (2002) [Refereed Article]
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The definitive published version is available online at: http://interscience.wiley.com
Official URL: http://interscience.wiley.com
DOI: doi:10.1002/1615-9314(20020901)25:13<819::AID-JSSC819>3.0.CO;2-6
Abstract
Methods based on capillary zone electrophoresis (CZE) and electrokinetic chromatography (EKC) are developed for the separation of motexafin gadolinium (MGd, Xcytrin®), a novel redox-active drug, under development for cancer therapy, and two closely related metallotexaphyrin impurities, called PCI-0400 and PCI-0430. The three metallotexaphyrins are baseline resolved using EKC in a separation solution containing 400-mM cetyltrimethyl ammonium bromide, 50-mM phosphoric acid, and 20% methanol. Detection is achieved using native laser-induced fluorescence (LIF) by exciting at 488 nm and collecting emission at 750 nm. Analysis of a representative clinical batch of MGd using EKC combined with native LIF allowed for the detection of PCI-0400 and PCI-0430 along with nine other possible MGd impurities, each of which is present at approximately 0.1% (total weight of the sample).
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | Expanded porphyrins • Metallotexaphyrins • Capillary electrophoresis • Electrokinetic chromatography • Motexafin gadolinium |
| Research Division: | Chemical Sciences |
| Research Group: | Analytical Chemistry |
| Research Field: | Separation Science |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding Knowledge |
| Objective Field: | Expanding Knowledge in the Chemical Sciences |
| Author: | Quirino, JP (Associate Professor Lito Quirino) |
| ID Code: | 54869 |
| Year Published: | 2002 |
| Web of Science® Times Cited: | 3 |
| Deposited By: | Austn Centre for Research in Separation Science |
| Deposited On: | 2009-03-02 |
| Last Modified: | 2011-11-16 |
| Downloads: | 0 |
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