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Capillary electrophoresis separation and native laser-induced fluorescence detection of metallotexaphyrins


Quirino, JP and Dulay, MT and Fu, L and Mody, TD and Zare, RN, Capillary electrophoresis separation and native laser-induced fluorescence detection of metallotexaphyrins, Journal of Separation Science, 25, (13) pp. 819-824. ISSN 1615-9314 (2002) [Refereed Article]

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DOI: doi:10.1002/1615-9314(20020901)25:13<819::AID-JSSC819>3.0.CO;2-6


Methods based on capillary zone electrophoresis (CZE) and electrokinetic chromatography (EKC) are developed for the separation of motexafin gadolinium (MGd, Xcytrin®), a novel redox-active drug, under development for cancer therapy, and two closely related metallotexaphyrin impurities, called PCI-0400 and PCI-0430. The three metallotexaphyrins are baseline resolved using EKC in a separation solution containing 400-mM cetyltrimethyl ammonium bromide, 50-mM phosphoric acid, and 20% methanol. Detection is achieved using native laser-induced fluorescence (LIF) by exciting at 488 nm and collecting emission at 750 nm. Analysis of a representative clinical batch of MGd using EKC combined with native LIF allowed for the detection of PCI-0400 and PCI-0430 along with nine other possible MGd impurities, each of which is present at approximately 0.1% (total weight of the sample).

Item Details

Item Type:Refereed Article
Keywords:Expanded porphyrins • Metallotexaphyrins • Capillary electrophoresis • Electrokinetic chromatography • Motexafin gadolinium
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:54869
Year Published:2002
Web of Science® Times Cited:5
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2009-03-02
Last Modified:2011-11-16

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