eCite Digital Repository
Capillary electrophoresis separation and native laser-induced fluorescence detection of metallotexaphyrins
Citation
Quirino, JP and Dulay, MT and Fu, L and Mody, TD and Zare, RN, Capillary electrophoresis separation and native laser-induced fluorescence detection of metallotexaphyrins, Journal of Separation Science, 25, (13) pp. 819-824. ISSN 1615-9314 (2002) [Refereed Article]
![]() | PDF Restricted - Request a copy 215Kb |
Copyright Statement
The definitive published version is available online at: http://interscience.wiley.com
Official URL: http://interscience.wiley.com
DOI: doi:10.1002/1615-9314(20020901)25:13<819::AID-JSSC819>3.0.CO;2-6
Abstract
Methods based on capillary zone electrophoresis (CZE) and electrokinetic chromatography (EKC) are developed for the separation of motexafin gadolinium (MGd, Xcytrin®), a novel redox-active drug, under development for cancer therapy, and two closely related metallotexaphyrin impurities, called PCI-0400 and PCI-0430. The three metallotexaphyrins are baseline resolved using EKC in a separation solution containing 400-mM cetyltrimethyl ammonium bromide, 50-mM phosphoric acid, and 20% methanol. Detection is achieved using native laser-induced fluorescence (LIF) by exciting at 488 nm and collecting emission at 750 nm. Analysis of a representative clinical batch of MGd using EKC combined with native LIF allowed for the detection of PCI-0400 and PCI-0430 along with nine other possible MGd impurities, each of which is present at approximately 0.1% (total weight of the sample).
Item Details
Item Type: | Refereed Article |
---|---|
Keywords: | Expanded porphyrins • Metallotexaphyrins • Capillary electrophoresis • Electrokinetic chromatography • Motexafin gadolinium |
Research Division: | Chemical Sciences |
Research Group: | Analytical Chemistry |
Research Field: | Separation Science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding Knowledge |
Objective Field: | Expanding Knowledge in the Chemical Sciences |
UTAS Author: | Quirino, JP (Associate Professor Lito Quirino) |
ID Code: | 54869 |
Year Published: | 2002 |
Web of Science® Times Cited: | 3 |
Deposited By: | Austn Centre for Research in Separation Science |
Deposited On: | 2009-03-02 |
Last Modified: | 2011-11-16 |
Downloads: | 0 |
Repository Staff Only: item control page