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Bonded-phase photopolymerized sol-gel monoliths for reversed phase capillary electrochromatography


Dulay, MT and Quirino, JP and Bennett, BD and Zare, RN, Bonded-phase photopolymerized sol-gel monoliths for reversed phase capillary electrochromatography, Journal of Separation Science, 25, (1-2) pp. 3-9. ISSN 1615-9314 (2001) [Refereed Article]

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DOI: doi:10.1002/1615-9314(20020101)25:1/2<3::AID-JSSC3>3.0.CO;2-L


A porous chemically modified photopolymerized sol-gel (PSG) monolith enhanced the capillary electrochromatographic separations of two test mixtures, one containing eight alkyl phenyl ketones and the other containing thiourea and three polyaromatic hydrocarbons. Derivatization of the PSG surface with silane coupling reagents resulted in bonded phases of pentafluorophenylpropyldimethyl, pentafluorophenyl, 3,3,3-trifluoropropyl, n-octadimethyl, perfluorohexyl, and aminopropyl. The fabrication of the bonded-phase PSG column is easy to do with the silanization reaction proceeding at room temperature for not longer than 60 minutes. The hydrophobicity of the PSG was altered without degrading its chromatographic performance. The bonded-phase PSG monoliths have higher stability at pH values below 4 as compared to the parent (underivatized) PSG. Separations of different mixtures containing nucleosides, positively charged peptides, and taxol derivatives illustrate the potential of bonded-phase PSG columns for the analyses of biologically and pharmaceutically important compounds. We report column efficiencies of up to 180 000 plates/meter and retention factors as large as 28.6 for decanophenone.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:54791
Year Published:2001
Web of Science® Times Cited:59
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2009-02-27
Last Modified:2011-11-16

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