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Thermal Unfolding of Proteins Studied by Coupled Reversed-Phase HPLC-Electrospray Ionization Mass Spectrometry Techniques Based on Isotope Exchange Effects
Citation
Hearn, MTW and Quirino, JP and Whisstock, J and Terabe, S, Thermal Unfolding of Proteins Studied by Coupled Reversed-Phase HPLC-Electrospray Ionization Mass Spectrometry Techniques Based on Isotope Exchange Effects, Analytical Chemistry, 74, (6) pp. 1467-1475. ISSN 0003-2700 (2002) [Refereed Article]
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Official URL: http://pubs.acs.org/
Abstract
In this study, a deuterium exchange procedure has been
employed to evaluate the thermal stability of globular
proteins under conditions that replicate their interactive
behavior in reversed-phase high performance chromatographic
(RP-HPLC) systems. In particular, this investigation
has permitted the conformational stability of two
proteins, hen egg white lysozyme (HEWL) and horse heart
myoglobin (HMYO) to be examined under different temperature
and low-pH solvent regimes. The results confirm
that this experimental approach provides an efficient
strategy to explore fundamental conformational features
of polypeptides or proteins in their folded and partial
unfolded states under these interactive conditions. In
particular, this analytical procedure permits insight to be
readily gained into the processes that occur when polypeptides
and globular proteins interact with lipophilic liquid/
solid interfaces in the presence of water-organic solvent
mixtures at different temperatures.
Item Details
Item Type: | Refereed Article |
---|---|
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Quirino, JP (Associate Professor Lito Quirino) |
ID Code: | 54785 |
Year Published: | 2002 |
Web of Science® Times Cited: | 8 |
Deposited By: | Austn Centre for Research in Separation Science |
Deposited On: | 2009-02-27 |
Last Modified: | 2012-11-19 |
Downloads: | 546 View Download Statistics |
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