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Development of a micro-fluidic manifold for copper monitoring utilising chemiluminescence detection


Tyrrell, EC and Gibson, C and MacCraith, BD and Gray, D and Byrne, P and Kent, N and Burke, C and Paull, B, Development of a micro-fluidic manifold for copper monitoring utilising chemiluminescence detection, Lab on a Chip, 4, (4) pp. 384-390. ISSN 1473-0197 (2004) [Refereed Article]

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Copyright 2004 Royal Society of Chemistry

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DOI: doi:10.1039/b400805g


The progressive development of a micro-fluidic manifold for the chemiluminescent detection of copper in water samples, based on the measurement of light emitted from the Cu(II) catalysed oxidation of 1,10-phenanthroline by hydrogen peroxide, is reported. Micro-fluidic manifolds were designed and manufactured from polymethylmethacrylate (PMMA) using three micro-fabrication techniques, namely hot embossing, laser ablation and direct micro-milling. The final laser ablated design incorporated a reagent mixing channel of dimensions 7.3 cm in length and 250 250 m in width and depth (triangular cross section), and a detection channel of 2.1 cm in length and 250 250 m in width and depth (total approx. volume of between 16 to 22 L). Optimised reagents conditions were found to be 0.07 mM 1,10-phenanthroline, containing 0.10 mM cetyltrimethylammonium bromide and 0.075 M sodium hydroxide (reagent 1 delivered at 0.025 mL min1) and 5% hydrogen peroxide (reagent 2 delivered at 0.025 mL min1). The sample stream was mixed with reagent 1 in the mixing channel and subsequently mixed with reagent 2 at the start of the detection channel. The laser ablated manifold was found to give a linear response (R2 = 0.998) over the concentration ranges 0150 g L1 and be reproducible (% RSD = 3.4 for five repeat injections of a 75 g L1 std). Detection limits for Cu(II) were found to be 20 g L1. Selectivity was investigated using a copper selective mini-chelating column, which showed common cations found in drinking waters did not cause interference with the detection of Cu(II). Finally the optimised system was successfully used for trace Cu(II) determinations in a standard reference freshwater sample (SRM 1640).

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Tyrrell, EC (Dr Eadaoin Tyrrell)
ID Code:54764
Year Published:2004
Web of Science® Times Cited:22
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2009-02-27
Last Modified:2009-07-27

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