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AtSERK1 expression precedes and coincides with early somatic embryogenesis in Arabidopsis thaliana


Salaj, J and von Recklinghausen, IR and Hecht, VFG and de Vries, SC and Schel, JHN and van Lammeren, AAM, AtSERK1 expression precedes and coincides with early somatic embryogenesis in Arabidopsis thaliana, Plant Physiology and Biochemistry, 46, (7) pp. 709-714. ISSN 0981-9428 (2008) [Refereed Article]

DOI: doi:10.1016/j.plaphy.2008.04.011


The Arabidopsis thaliana primordia timing (pt) mutant was transformed with an AtSERK1::GUS construct. Liquid cultures of this line were used to study the relationship between somatic embryogenesis and the expression of SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (AtSERK1) as a marker for cells competent to form embryos. In order to search for the expression of AtSERK1::GUS during early stages of somatic embryogenesis, histochemical as well as immunochemical approaches were used for the detection of β-glucuronidase (GUS). Four sites of AtSERK1 expression were found in the embryogenic cultures: in embryogenic callus, where primary somatic embryos developed; in the basal parts of primary somatic embryos; in the outer layers of cotyledons of primary somatic embryos where secondary embryos were formed; and in provascular and vascular strands of developing somatic embryos. The in vitro expression of AtSERK1::GUS coincides with embryogenic development up to the heart-shaped stage. Prior to the expression in embryos, AtSERK1 was expressed in single cells and small cell clusters, indicating that AtSERK1 indeed marks embryogenic competence. Its expression in (pro)vascular strands, suggests that embryogenic cells in tissue culture retain at least in part their original identity.

Item Details

Item Type:Refereed Article
Keywords:Antibodies; Arabidopsis; AtSERK1; GUS; SERK; Somatic embryogenesis; Suspension culture
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant developmental and reproductive biology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural crops
Objective Field:Horticultural crops not elsewhere classified
UTAS Author:Hecht, VFG (Dr Valerie Hecht)
ID Code:54701
Year Published:2008
Web of Science® Times Cited:33
Deposited By:Plant Science
Deposited On:2009-02-27
Last Modified:2009-05-18

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