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Discrimination between seedlings of Eucalyptus globulus, E. nitens and their F1 hybrid using near-infrared reflectance spectroscopy and foliar oil content

Citation

Humphreys, JR and O'Reilly-Wapstra, JM and Harbard, JL and Davies, NW and Griffin, AR and Jordan, GJ and Potts, BM, Discrimination between seedlings of Eucalyptus globulus, E. nitens and their F1 hybrid using near-infrared reflectance spectroscopy and foliar oil content, Silvae Genetica, 57, (4-5) pp. 262-269. ISSN 0037-5349 (2008) [Refereed Article]

DOI: doi:10.1515/sg-2008-0040

Abstract

Identification of plant hybrids produced from closely related species can be difficult using morphological characteristics alone, particularly when identifying young seedlings. In this study, we compared the performance of three calibration models developed to discriminate between seedlings of Eucalyptus globulus, E. nitens and their first-generation hybrid using either foliar oil chemistry or near-infrared reflectance spectral data from fresh, whole leaves. Both oil and near-infrared reflectance spectroscopy (NIRS) models were developed using partial least-squares discriminant analysis and showed high classification accuracy, all correctly classifying more than 91% of samples in cross-validation. Additionally, we developed a larger, "global" and independently validated NIRS model specifically to discriminate between E. globulus and F1 hybrid seedlings of different ages. This model correctly classified 98.1% of samples in cross-validation and 95.1% of samples from an independent test set. These results show that NIRS analysis of fresh, whole leaves can be used as a rapid and accurate alternative to chemical analysis for the purpose of hybrid identification.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Forestry Sciences
Research Field:Forestry Management and Environment
Objective Division:Plant Production and Plant Primary Products
Objective Group:Forestry
Objective Field:Hardwood Plantations
Author:Humphreys, JR (Mr Jonathan Humphreys)
Author:O'Reilly-Wapstra, JM (Dr Julianne O'Reilly-Wapstra)
Author:Harbard, JL (Ms Jane Harbard)
Author:Davies, NW (Associate Professor Noel Davies)
Author:Griffin, AR (Professor Rod Griffin)
Author:Jordan, GJ (Associate Professor Greg Jordan)
Author:Potts, BM (Professor Brad Potts)
ID Code:54644
Year Published:2008
Web of Science® Times Cited:11
Deposited By:Plant Science
Deposited On:2009-02-26
Last Modified:2015-02-07
Downloads:0

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