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Iron availability regulates growth, photosynthesis, and production of ferredoxin and flavodoxin in Antarctic sea ice diatoms


Pankowski, A and McMinn, A, Iron availability regulates growth, photosynthesis, and production of ferredoxin and flavodoxin in Antarctic sea ice diatoms, Aquatic Biology, 4, (3) pp. 273-288. ISSN 1864-7790 (2009) [Refereed Article]


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Inter-Research 2009

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DOI: doi:10.3354/ab00116


Iron availability affects the growth of not only phytoplankton, but also sea ice diatoms. Iron availability had a clear effect on the growth rates of Fragilariopsis cylindrus and F. curta. Maximum growth rates were 0.57 dC1 for F. cylindrus and 0.28 dC1 for F. curta; Km (half-saturation growth constant) was 0.51 〜 10C12 and 1.3 〜 10C12 M for F. cylindrus and F. curta, respectively. For both F. cylindrus and F. curta, Fv/Fm (quantum yield of fluorescence) was highest and lowest for the cultures grown with the highest and lowest concentrations of iron, respectively. For both species there was also a reduction in both rETRmax (maximum relative electron transfer rate) and α (photosynthetic efficiency) with decreasing iron concentration. For F. cylindrus grown with the least iron, rETRmax was half of the iron-replete value, while α was reduced by 65%. Changes in Ek (light-adaptation parameter) were not well defined. Immunoassays were developed for the proteins ferredoxin and flavodoxin in Antarctic pack ice. Iron availability had different effects on the expression of flavodoxin and ferredoxin in the 2 Fragilariopsis species tested. Cultures of F. cylindrus grown at high iron concentration produced predominantly ferredoxin, with a small amount of flavodoxin. Ferredoxin was sequentially replaced by flavodoxin for cultures grown with less iron, although the response was not a simple switch from one protein to the other. The ability to produce ferredoxin is apparently absent in F. curta, with relatively constant levels of flavodoxin produced at all iron concentrations. These results strongly imply that the presence of flavodoxin alone cannot be used as evidence of ironlimited growth.

Item Details

Item Type:Refereed Article
Keywords:Antarctic , Sea ice , Iron , Ferredoxin , Flavodoxin , Microalgae
Research Division:Biological Sciences
Research Group:Ecology
Research Field:Marine and estuarine ecology (incl. marine ichthyology)
Objective Division:Environmental Management
Objective Group:Terrestrial systems and management
Objective Field:Assessment and management of terrestrial ecosystems
UTAS Author:Pankowski, A (Mr Andrew Pankowski)
UTAS Author:McMinn, A (Professor Andrew McMinn)
ID Code:54382
Year Published:2009
Web of Science® Times Cited:28
Deposited By:IASOS
Deposited On:2009-02-19
Last Modified:2010-05-07
Downloads:442 View Download Statistics

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