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In vitro Effect of Garlic Extract and Metronidazole Against Neoparamoeba pemaquidensis, Page 1987 and Isolated Amoebae from Atlantic Salmon

Citation

Peyghan, R and Powell, MD and Zadkarami, MR, In vitro Effect of Garlic Extract and Metronidazole Against Neoparamoeba pemaquidensis, Page 1987 and Isolated Amoebae from Atlantic Salmon, Pakistan Journal of Biological Sciences, 11, (1) pp. 41-47. ISSN 1028-8880 (2008) [Refereed Article]


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DOI: doi:10.3923/pjbs.2008.41.47

Abstract

Neoparamoeba pemaquidensis believed to be the most prevalent parasite of Atlantic salmon industry in Australia. In the present study, the in vitro effects of crude extract of garlic and metronidazole were investigated using a primary culture toxicity assay. Garlic extract appeared to be completely effective at killing a cultured strain (NP251002) of Neoparamoeba pemaquidensis in vitro at a dilution of 1:100 with in 24 h. The number of viable Amoebae after using garlic extract in lower dilutions (1:200, 1:400, 1:800, 1:1000) for 24 h, also were significantly lower than in the control group. Garlic extract was also efficacious at killing wild type Amoebae that isolated from the diseased fish showing clinical signs of AGD. Metronidazole had no clear effect against Neoparamoeba pemaquidensis (NP251002) even in a concentration of 50 mg L-1 for 24 h. However some morphological changes have occurred in metronidazole-treated Amoebae after 5 days of exposure

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Fin Fish (excl. Tuna)
Author:Powell, MD (Dr Mark Powell)
ID Code:54348
Year Published:2008
Deposited By:NC Marine Conservation and Resource Sustainability
Deposited On:2009-02-18
Last Modified:2009-11-05
Downloads:583 View Download Statistics

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