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A microsatellite study on outcrossing rates and contamination in an Eucalyptus globulus breeding arboretum


RAO, H and Patterson, B and Potts, BM and Vaillancourt, RE, A microsatellite study on outcrossing rates and contamination in an Eucalyptus globulus breeding arboretum, Journal of Forestry Research, 19, (2) pp. 136-140. ISSN 1007-662X (2008) [Refereed Article]

DOI: doi:10.1007/s11676-008-0023-6


Four pairs of microsatellite molecular polymorphism primers were used to analyse microsatellite fingerprints of 188 seedlings derived from an open-pollinated progeny grafted Eucalyptus globulus breeding arboretum in Victoria, south-eastern Australia. The microsatellite loci chosen for this study were highly polymorphic with the mean number of alleles per locus of 14.25. Individual mothers varied in their outcrosssing rate estimate from 15% to 95%, the overall outcrossing level in the arboretum was 47.9% and the contamination rate was 17.6%. The high selfing level was likely to result in marked inbreeding depression in the performance of open-pollinated seed lots. Open-pollinated seeds collected from such arboreta are not advisable because of its low genetic quality, although such arboreta may be useful for the seed production through large-scale manual pollination or collecting seeds only from trees or genotypes within the arboretum that have high outcrossing rates.

Item Details

Item Type:Refereed Article
Keywords:E. globulus - breeding arboretum - microsatellite - outcrossing rate - pollen contamination
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Forestry sciences
Research Field:Tree improvement (incl. selection and breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Forestry
Objective Field:Hardwood plantations
UTAS Author:Patterson, B (Dr Briony Patterson)
UTAS Author:Potts, BM (Professor Brad Potts)
UTAS Author:Vaillancourt, RE (Professor Rene Vaillancourt)
ID Code:54154
Year Published:2008
Deposited By:Plant Science
Deposited On:2009-02-11
Last Modified:2011-11-02

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