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Study of New Extraction Methods for Separation of Anthocyanins from Red Grape Skins: Analysis by HPLC and LC-MS/MS


Ghassempour, A and Heydari, R and Talebpour, Z and Fakhari, AR and Rassouli, A and Davies, NW and Aboul-Enein, HY, Study of New Extraction Methods for Separation of Anthocyanins from Red Grape Skins: Analysis by HPLC and LC-MS/MS, Journal of Liquid Chromatography & Related Technologies, 31, (17) pp. 2686-2703. ISSN 1082-6076 (2008) [Refereed Article]

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DOI: doi:10.1080/10826070802353247


Microwave assisted extraction (MAE) and ultrasound assisted extraction (UAE) methods were applied for the first time for the extraction of anthocyanins from red grape skins, and the results of these methods were compared with the maceration method. The extracted samples were analyzed by high performance liquid chromatography (HPLC) and nine anthocyanins were identified by liquid chromatography tandem mass spectrometry (LC-MS=MS). The effects of sample weight to solvent volume ratio, temperature, and time of extraction on the efficiency of extraction in MAE were investigated by two full factorial designs at three levels. The results of two designs show that sample weight to solvent volume ratio and temperature were more important than the time of extraction in MAE. UAE has no variable parameters similar to MAE. The suitable solvent and required time for the UAE method were described. The recoveries of MAE, UAE, and maceration methods were 90%, 82%, and 98%, respectively.

Item Details

Item Type:Refereed Article
Keywords:Anthocyanin, Grape skin, High performance liquid chromatography (HPLC), Microwave-assisted extraction (MAE), Tandem mass spectrometry (MS-MS), Ultrasound assisted extraction (UAE)
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Instrumental methods (excl. immunological and bioassay methods)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural crops
Objective Field:Table grapes
UTAS Author:Davies, NW (Associate Professor Noel Davies)
ID Code:53749
Year Published:2008
Web of Science® Times Cited:34
Deposited By:Central Science Laboratory
Deposited On:2008-12-29
Last Modified:2009-11-11
Downloads:3 View Download Statistics

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