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Study of New Extraction Methods for Separation of Anthocyanins from Red Grape Skins: Analysis by HPLC and LC-MS/MS
Citation
Ghassempour, A and Heydari, R and Talebpour, Z and Fakhari, AR and Rassouli, A and Davies, NW and Aboul-Enein, HY, Study of New Extraction Methods for Separation of Anthocyanins from Red Grape Skins: Analysis by HPLC and LC-MS/MS, Journal of Liquid Chromatography & Related Technologies, 31, (17) pp. 2686-2703. ISSN 1082-6076 (2008) [Refereed Article]
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The definitive published version is available online at: http://www.tandf.co.uk/journals
Official URL: http://www.tandf.co.uk/journals
DOI: doi:10.1080/10826070802353247
Abstract
Microwave assisted extraction (MAE) and ultrasound assisted extraction
(UAE) methods were applied for the first time for the extraction of anthocyanins
from red grape skins, and the results of these methods were compared
with the maceration method. The extracted samples were analyzed by high performance liquid chromatography (HPLC) and nine anthocyanins were identified
by liquid chromatography tandem mass spectrometry (LC-MS=MS). The effects
of sample weight to solvent volume ratio, temperature, and time of extraction
on the efficiency of extraction in MAE were investigated by two full factorial
designs at three levels. The results of two designs show that sample weight to
solvent volume ratio and temperature were more important than the time
of extraction in MAE. UAE has no variable parameters similar to MAE. The
suitable solvent and required time for the UAE method were described. The
recoveries of MAE, UAE, and maceration methods were 90%, 82%, and
98%, respectively.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | Anthocyanin, Grape skin, High performance liquid chromatography (HPLC), Microwave-assisted extraction (MAE), Tandem mass spectrometry (MS-MS), Ultrasound assisted extraction (UAE) |
| Research Division: | Chemical Sciences |
| Research Group: | Analytical chemistry |
| Research Field: | Instrumental methods (excl. immunological and bioassay methods) |
| Objective Division: | Plant Production and Plant Primary Products |
| Objective Group: | Horticultural crops |
| Objective Field: | Table grapes |
| UTAS Author: | Davies, NW (Associate Professor Noel Davies) |
| ID Code: | 53749 |
| Year Published: | 2008 |
| Web of Science® Times Cited: | 31 |
| Deposited By: | Central Science Laboratory |
| Deposited On: | 2008-12-29 |
| Last Modified: | 2009-11-11 |
| Downloads: | 3 View Download Statistics |
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