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Genes implicated in multiple sclerosis pathogenesis fromconsilience of genotyping and expression profiles in relapse and remission

Citation

Arthur, AT and Armati, PJ and Bye, C and Kilpatrick, TJ and Foote, SJ and Butzkueven, H and Taylor, BVM and Tubridy, N and Marriott, M and Chapman, C and Bahlo, M and Speed, TP and Stankovich, J and Heard, RNS and Stewart, GJ and Pollard, JD and Booth, DR, Genes implicated in multiple sclerosis pathogenesis fromconsilience of genotyping and expression profiles in relapse and remission, BMC Medical Genetics, 9, (Mar 19) pp. 17. ISSN 1471-2350 (2008) [Refereed Article]

DOI: doi:10.1186/1471-2350-9-17

Abstract

Background: Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system (CNS). Although the pathogenesis of MS remains unknown, it is widely regarded as an autoimmune disease mediated by T-lymphocytes directed against myelin proteins and/or other oligodendrocyte epitopes. Methods: In this study we investigated the gene expression profiles of peripheral blood cells from patients with RRMS during the relapse and the remission phases utilizing gene microarray technology. Dysregulated genes encoded in regions associated with MS susceptibility from genomic screens or previous trancriptomic studies were identified. The proximal promoter region polymorphisms of two genes were tested for association with disease and expression level. Results: Distinct sets of dysregulated genes during the relapse and remission phases were identified including genes involved in apoptosis and inflammation. Three of these dysregulated genes have been previously implicated with MS susceptibility in genomic screens: TGFβ1, CD58 and DBC1. TGFβ1 has one common SNP in the proximal promoter: -508 T>C (rs1800469). Genotyping two Australian trio sets (total 620 families) found a trend for over-transmission of the T allele in MS in females (p < 0.13). Upregulation of CD58 and DBC1 in remission is consistent with their putative roles in promoting regulatory T cells and reducing cell proliferation, respectively. A fourth gene, ALOX5, is consistently found over-expressed in MS. Two common genetic variants were confirmed in the ALOX5 putatve promoter: -557 T>C (rs12762303) and a 6 bp tandem repeat polymorphism (GGGCGG) between position -147 and -176; but no evidence for transmission distortion found. Conclusion: The dysregulation of these genes tags their metabolic pathways for further investigation for potential therapeutic intervention. © 2008 Arthur et al; licensee BioMed Central Ltd.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Neurology and Neuromuscular Diseases
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Inherited Diseases (incl. Gene Therapy)
UTAS Author:Foote, SJ (Professor Simon Foote)
UTAS Author:Taylor, BVM (Professor Bruce Taylor)
UTAS Author:Stankovich, J (Dr Jim Stankovich)
ID Code:53598
Year Published:2008
Web of Science® Times Cited:46
Deposited By:Menzies Institute for Medical Research
Deposited On:2008-12-16
Last Modified:2009-05-28
Downloads:0

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