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Mitogen-induced responses in lymphocytes from platypus, the Tasmanian devil and the eastern barred bandicoot

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posted on 2023-05-16, 22:20 authored by Niall Stewart, Silvana BettiolSilvana Bettiol, Kreiss, A, Fox, N, Gregory WoodsGregory Woods
Objective: As the platypus (Ornithorhynchus anatinus), the Tasmanian devil (Sarcophilus harrisi) and the eastern barred bandicoot (Perameles gunni) are currently at risk of serious population decline or extinction from fatal diseases in Tasmania, the goal of the present study was to describe the normal immune response of these species to challenge using the lymphocyte proliferation assay, to give a solid basis for further studies. Methods: For this preliminary study, we performed lymphocyte proliferation assays on peripheral blood mononuclear cells (PBMC) from the three species. We used the common mitogens phytohaemagglutinin (PHA), concanavalin A (ConA), lipopolysaccharide (LPS) and pokeweed mitogen (PWM). Results: All three species recorded the highest stimulation index (SI) with the T-cell mitogens PHA and ConA. Tasmanian devils and bandicoots had greater responses than platypuses, although variability between individual animals was high. Conclusion: For the first time, we report the normal cellular response of the platypus, the Tasmanian devil and the eastern barred bandicoot to a range of commonly used mitogens. © 2008 Australian Veterinary Association.

History

Publication title

Australian Veterinary Journal

Volume

86

Issue

10

Pagination

408-413

ISSN

0005-0423

Department/School

Menzies Institute for Medical Research

Publisher

Wiley-Blackwell Publishing LTd

Place of publication

United Kingdom

Repository Status

  • Restricted

Socio-economic Objectives

Veterinary pharmaceutical products not elsewhere classified

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