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Mitogen-induced responses in lymphocytes from platypus, the Tasmanian devil and the eastern barred bandicoot

Citation

Stewart, NJ and Bettiol, SS and Kreiss, A and Fox, N and Woods, GM, Mitogen-induced responses in lymphocytes from platypus, the Tasmanian devil and the eastern barred bandicoot, Australian Veterinary Journal, 86, (10) pp. 408-413. ISSN 0005-0423 (2008) [Refereed Article]

DOI: doi:10.1111/j.1751-0813.2008.00349.x

Abstract

Objective: As the platypus (Ornithorhynchus anatinus), the Tasmanian devil (Sarcophilus harrisi) and the eastern barred bandicoot (Perameles gunni) are currently at risk of serious population decline or extinction from fatal diseases in Tasmania, the goal of the present study was to describe the normal immune response of these species to challenge using the lymphocyte proliferation assay, to give a solid basis for further studies. Methods: For this preliminary study, we performed lymphocyte proliferation assays on peripheral blood mononuclear cells (PBMC) from the three species. We used the common mitogens phytohaemagglutinin (PHA), concanavalin A (ConA), lipopolysaccharide (LPS) and pokeweed mitogen (PWM). Results: All three species recorded the highest stimulation index (SI) with the T-cell mitogens PHA and ConA. Tasmanian devils and bandicoots had greater responses than platypuses, although variability between individual animals was high. Conclusion: For the first time, we report the normal cellular response of the platypus, the Tasmanian devil and the eastern barred bandicoot to a range of commonly used mitogens. © 2008 Australian Veterinary Association.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Zoology
Research Field:Animal Immunology
Objective Division:Manufacturing
Objective Group:Veterinary Pharmaceutical Products
Objective Field:Veterinary Pharmaceutical Products not elsewhere classified
UTAS Author:Stewart, NJ (Dr Niall Stewart)
UTAS Author:Bettiol, SS (Dr Silvana Bettiol)
UTAS Author:Kreiss, A (Dr Alexandre Kreiss)
UTAS Author:Woods, GM (Professor Gregory Woods)
ID Code:53573
Year Published:2008
Web of Science® Times Cited:5
Deposited By:Menzies Institute for Medical Research
Deposited On:2008-12-12
Last Modified:2009-05-02
Downloads:0

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