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A rapid flow cytometric technique for the detection of platelet-monocyte complexes, activated platelets and platelet-derived microparticles

Citation

Pearson, L and Thom, J and Adams, MJ and Oostryck, R and Krueger, R and Yong, G and Baker, R, A rapid flow cytometric technique for the detection of platelet-monocyte complexes, activated platelets and platelet-derived microparticles, International Journal of Laboratory Hematology, 31, (4) pp. 430-439. ISSN 1751-5521 (2009) [Refereed Article]


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Copyright Statement

The definitive published version is available online at: http://interscience.wiley.com

Official URL: http://interscience.wiley.com

DOI: doi:10.1111/j.1751-553X.2008.01059.x

Abstract

Platelet activation occurs in a variety of clinical situations in which it directly contributes to the pathology. This study reports a simple flow cytometric assay for platelet activation which measures platelet-derived microparticles, activated platelets and platelet–monocyte complexes. Pre- and post analytical conditions were investigated and optimized and a normal range established on 20 healthy controls. Twenty patients pre- and post percutaneous coronary intervention (PCI) were tested with the technique. Soluble activation markers sCD40 ligand and sP-selectin and plasma phospholipid levels were measured in both groups. There was a significant increase in activated platelets and platelet–monocyte complexes between normal and pre-PCI (P = 0.005 and 0.0275, respectively) suggesting an activated state. There was a significant fall in activated platelets post-PCI (P = 0.0027) which was mirrored by a fall in soluble CD40 ligand, soluble P-selectin and plasma phospholipid levels (P = 0.0066, <0.0001 and 0.0032, respectively) consistent with antiplatelet therapy administered during the process. This is a reliable and rapid method for the assessment of ex vivo platelet activation which may be an aid in diagnosis and help guide therapy for patients with thrombotic disease.

Item Details

Item Type:Refereed Article
Keywords:microparticles, haematology, platelet monocyte factor
Research Division:Medical and Health Sciences
Research Group:Cardiorespiratory Medicine and Haematology
Research Field:Haematology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Blood Disorders
UTAS Author:Adams, MJ (Dr Murray Adams)
ID Code:53366
Year Published:2009
Web of Science® Times Cited:13
Deposited By:Health Sciences A
Deposited On:2008-12-03
Last Modified:2011-07-27
Downloads:3 View Download Statistics

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