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Investigation of the effect of heating on the chemistry and antifactor Xa activity of enoxaparin

Citation

Patel, R and Narkowicz, CK and Jacobson, GA, Investigation of the effect of heating on the chemistry and antifactor Xa activity of enoxaparin, Journal of Pharmaceutical Science, 98, (5) pp. 1700 - 1711. ISSN 0022-3549 (2009) [Refereed Article]


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Copyright Statement

The definitive published version is available online at: http://interscience.wiley.com

Official URL: http://interscience.wiley.com

DOI: doi:10.1002/jps.21556

Abstract

The objective of this study was to investigate the effects of heating on the chemistry, physical properties and antifactor Xa activity of enoxaparin. Samples of enoxaparin heated at 70°C lost 27% of their initial AFXa activity after 8 h, then activity increased to 94% of the initial activity over the next 4 h. Activity then decreased to 84% of control after 48 h and further to 80% of control over 22 days. The initial activity loss correlated with desulfation as demonstrated by sulfate and amine analysis. Fragmentation of oligosaccharides occurred, as demonstrated by reducing capacity and capillary electrophoresis analysis. Individual enoxaparin fractions obtained by high performance size exclusion chromatography were analysed. Early eluting fractions, containing aggregated oligosaccharides, increased in concentration following heating. Up to 65% of sulfate was lost from some fractions, containing hexa- and octa-saccharides, after 8 h, corresponding with decreased activity. Low mass oligosaccharide fractions increased in concentration and had increased activity between 8 and 12 h. Reversed-phase ion-interaction HPLC analysis supported these findings. Deca-, dodeca- and tetradeca-saccharides were resistant to thermal degradation. Desulfation, fragmentation and aggregation occur during the heating of enoxaparin and result in the initial rapid loss, recovery and subsequent gradual loss of activity.

Item Details

Item Type:Refereed Article
Keywords:AFXa activity • enoxaparin • stability • heating • sulfate • fragmentation • aggregation
Research Division:Medical and Health Sciences
Research Group:Pharmacology and Pharmaceutical Sciences
Research Field:Pharmaceutical Sciences
Objective Division:Health
Objective Group:Public Health (excl. Specific Population Health)
Objective Field:Public Health (excl. Specific Population Health) not elsewhere classified
UTAS Author:Patel, R (Dr Rahul Patel)
UTAS Author:Narkowicz, CK (Dr Christian Narkowicz)
UTAS Author:Jacobson, GA (Associate Professor Glenn Jacobson)
ID Code:53238
Year Published:2009
Web of Science® Times Cited:13
Deposited By:Pharmacy
Deposited On:2008-11-14
Last Modified:2010-06-29
Downloads:5 View Download Statistics

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