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Association of serotonin, dopamine, or noradrenaline with an actin-like component in pheochromocytoma (PC12) cells
Abstract: A rat pheochromocytoma (PC12) cell line was used to examine the possibility that 5â€hydroxytryptamine (serotonin), 3,4â€dihydroxyphenylethylamine (dopamine), or noradrenaline may be associated with cytoplasmic actin, as was suggested by previous in vitro binding studies on an actinâ€like protein from rat brain synaptosomes. When PC12 cells were incubated with [3H]serotonin, [3H]dopamine, or [3H]noradrenaline for 30 min at 37°C, approximately 2–4% of the radioactivity present in the cells was found to be associated with a highâ€molecularâ€weight (actinâ€like) component in supernatant fractions. Evidence relating this monoamine binding component to actin filaments includes: (a) its strong absorption by myosin filaments at low ionic strength; (b) a decrease in its affinity for myosin in the presence of 1 mM ATP, which lowers the affinity of authentic actin for myosin; (c) displacement of bound [3H]serotonin from it by DNase I, which binds strongly to actin and which inhibits [3H]serotonin binding to actin in vitro; (d) an increase in its binding of each monoamine (by 25–40%) after PC12 cells were preincubated with 10 μM cytochalasin B (a drug that induces depolymerization of Fâ€actin). These findings suggest that serotonin, dopamine, or noradrenaline may associate with actin filaments in vivo. Copyright © 1985, Wiley Blackwell. All rights reserved
History
Publication title
Journal of NeurochemistryVolume
45Pagination
825-831ISSN
0022-3042Department/School
Menzies Institute for Medical ResearchPublisher
Wiley-Blackwell Publishing Ltd.Place of publication
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