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Association of serotonin, dopamine, or noradrenaline with an actin-like component in pheochromocytoma (PC12) cells


Small, DH and Wurtman, RJ, Association of serotonin, dopamine, or noradrenaline with an actin-like component in pheochromocytoma (PC12) cells, Journal of Neurochemistry, 45, (3) pp. 825-831. ISSN 0022-3042 (1985) [Refereed Article]

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DOI: doi:10.1111/j.1471-4159.1985.tb04068.x


Abstract: A rat pheochromocytoma (PC12) cell line was used to examine the possibility that 5‐hydroxytryptamine (serotonin), 3,4‐dihydroxyphenylethylamine (dopamine), or noradrenaline may be associated with cytoplasmic actin, as was suggested by previous in vitro binding studies on an actin‐like protein from rat brain synaptosomes. When PC12 cells were incubated with [3H]serotonin, [3H]dopamine, or [3H]noradrenaline for 30 min at 37°C, approximately 2"4% of the radioactivity present in the cells was found to be associated with a high‐molecular‐weight (actin‐like) component in supernatant fractions. Evidence relating this monoamine binding component to actin filaments includes: (a) its strong absorption by myosin filaments at low ionic strength; (b) a decrease in its affinity for myosin in the presence of 1 mM ATP, which lowers the affinity of authentic actin for myosin; (c) displacement of bound [3H]serotonin from it by DNase I, which binds strongly to actin and which inhibits [3H]serotonin binding to actin in vitro; (d) an increase in its binding of each monoamine (by 25"40%) after PC12 cells were preincubated with 10 μM cytochalasin B (a drug that induces depolymerization of F‐actin). These findings suggest that serotonin, dopamine, or noradrenaline may associate with actin filaments in vivo. Copyright © 1985, Wiley Blackwell. All rights reserved

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Neurosciences not elsewhere classified
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Small, DH (Professor David Small)
ID Code:52872
Year Published:1985
Web of Science® Times Cited:7
Deposited By:Menzies Institute for Medical Research
Deposited On:2008-10-03
Last Modified:2012-02-22

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