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Olfactory ensheathing cells are attracted to, and can endocytose, bacteria


Leung, JY and Chapman, JA and Harris, JA and Hale, DM and Chung, RS and West, AK and Chuah, MI, Olfactory ensheathing cells are attracted to, and can endocytose, bacteria, Cellular and Molecular Life Sciences, 65, (17) pp. 2732-2739. ISSN 1420-682X (2008) [Refereed Article]

DOI: doi:10.1007/s00018-008-8184-1


Olfactory ensheathing cells (OECs) have been shown previously to express Toll-like receptors and to respond to bacteria by translocating nuclear factor-kB from the cytoplasm to the nucleus. In this study,we show that OECs extended significantly more pseudopodia when they were exposed to Escherichia coli than in the absence of bacteria (p=0.019). Coimmunoprecipitation showed that E. coli binding to OECs was mediated by Toll-like receptor 4. Lyso-Tracker, a fluorescent probe that accumulates selectively in lysosomes, and staining for type 1 lysosomeassociated membrane proteins demonstrated that endocytosed FITC-conjugated E. coli were translocated to lysosomes. They appeared to be subsequently broken down, as shown by transmission electron microscopy. No obvious adherence to the membrane and less phagocytosis was observed when OECs were incubated with inert fluorescent microspheres. The ability of OECs to endocytose bacteria supports the notion that OECs play an innate immune function by protecting olfactory tissues from bacterial infection.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Peripheral nervous system
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the environmental sciences
UTAS Author:Leung, JY (Dr Jacqueline Leung)
UTAS Author:Chapman, JA (Dr Jamie Chapman)
UTAS Author:Harris, JA (Dr Julie Harris)
UTAS Author:Hale, DM (Mr David Hale)
UTAS Author:Chung, RS (Associate Professor Roger Chung)
UTAS Author:West, AK (Professor Adrian West)
UTAS Author:Chuah, MI (Associate Professor Inn Chuah)
ID Code:52803
Year Published:2008
Web of Science® Times Cited:56
Deposited By:Menzies Institute for Medical Research
Deposited On:2008-09-16
Last Modified:2015-02-05

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