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Pilosulin 5, a novel histamine-releasing peptide of the Australian ant, Myrmecia pilosula (Jack Jumper Ant)
journal contribution
posted on 2023-05-16, 22:02 authored by Inagaki, H, Agaki, M, Imai, HT, Taylor, RW, Wiese, M, Noel DaviesNoel Davies, Kubo, TVenom of the Australian ant species Myrmecia pilosula contains a number of allergenic peptides including pilosulins. To obtain novel cDNA clones that encode the pilosulin-related bioactive peptides, mRNA of M. pilosula species complex was subjected to RT-PCR in which the forward primer corresponds to a nucleotide sequence in the leader sequences of pilosulins. We isolated a cDNA clone encoding the novel bioactive peptide pilosulin 5. Tandem mass analysis was entirely consistent with the cDNA derived sequence, and indicated that pilosulin 5 is connected by a single disulfide bridge to create a dimmer peptide of 8546 Da. Synthetic pilosulin 5 peptide caused a significant histamine release in a dose-dependent manner, and the mastoparan homologous region of pilosulin 5 was responsible for the activity
History
Publication title
Archives of Biochemistry and BiophysicsVolume
477Pagination
411-416ISSN
0003-9861Department/School
Menzies Institute for Medical ResearchPublisher
ElsevierPlace of publication
AmsterdamRights statement
The definitive version is available at http://www.sciencedirect.comRepository Status
- Restricted