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Pilosulin 5, a novel histamine-releasing peptide of the Australian ant, Myrmecia pilosula (Jack Jumper Ant)

Citation

Inagaki, H and Agaki, M and Imai, HT and Taylor, RW and Wiese, M and Davies, NW and Kubo, T, Pilosulin 5, a novel histamine-releasing peptide of the Australian ant, Myrmecia pilosula (Jack Jumper Ant), Archives of Biochemistry and Biophysics, 477, (2) pp. 411-416. ISSN 0003-9861 (2008) [Refereed Article]


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DOI: doi:10.1016/j.abb.2008.05.014

Abstract

Venom of the Australian ant species Myrmecia pilosula contains a number of allergenic peptides including pilosulins. To obtain novel cDNA clones that encode the pilosulin-related bioactive peptides, mRNA of M. pilosula species complex was subjected to RT-PCR in which the forward primer corresponds to a nucleotide sequence in the leader sequences of pilosulins. We isolated a cDNA clone encoding the novel bioactive peptide pilosulin 5. Tandem mass analysis was entirely consistent with the cDNA derived sequence, and indicated that pilosulin 5 is connected by a single disulfide bridge to create a dimmer peptide of 8546 Da. Synthetic pilosulin 5 peptide caused a significant histamine release in a dose-dependent manner, and the mastoparan homologous region of pilosulin 5 was responsible for the activity

Item Details

Item Type:Refereed Article
Keywords:Ant, Pilosulin, Myrmecia pilosula, Histamine-releasing activity
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Allergy
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Immune System and Allergy
UTAS Author:Wiese, M (Associate Professor Michael Wiese)
UTAS Author:Davies, NW (Associate Professor Noel Davies)
ID Code:52786
Year Published:2008
Web of Science® Times Cited:25
Deposited By:Central Science Laboratory
Deposited On:2008-08-01
Last Modified:2009-11-11
Downloads:0

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