eCite Digital Repository

A molecular approach to identify prey of the southern rock lobster

Citation

Redd, KS and Jarman, SN and Frusher, SD and Johnson, CR, A molecular approach to identify prey of the southern rock lobster, Bulletin of Entomological Research, 98, (3) pp. 233-238. ISSN 0007-4853 (2008) [Refereed Article]

DOI: doi:10.1017/S0007485308005981

Abstract

We demonstrate the use of molecular techniques to detect specific prey consumed by the southern rock lobster (Jasus edwardsii). A quick and non-lethal method was used to collect rock lobster faecal material and a molecular protocol was employed to isolate prey DNA from faecal samples. The isolated DNA was amplified using the polymerase chain reaction (PCR) with PCR primers designed to target specific prey items. Feeding experiments determined that DNA from black-lipped abalone (Haliotis rubra) and sea urchins (Centrostephanus rodgersii and Heliocidaris erythrogramma) can be detected in rock lobster faecal samples within seven hours and remains present for up to 60 h after ingestion. Copyright © 2008 Cambridge University Press.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Zoology
Research Field:Invertebrate Biology
Objective Division:Environment
Objective Group:Flora, Fauna and Biodiversity
Objective Field:Marine Flora, Fauna and Biodiversity
Author:Redd, KS (Mr Kevin Redd)
Author:Frusher, SD (Professor Stewart Frusher)
Author:Johnson, CR (Professor Craig Johnson)
ID Code:52289
Year Published:2008
Web of Science® Times Cited:10
Deposited By:TAFI - Zoology
Deposited On:2008-06-16
Last Modified:2011-09-27
Downloads:0

Repository Staff Only: item control page