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Diagnostic assays and sampling protocols for the detection of Batrachochytrium dendrobatidis

journal contribution
posted on 2023-05-16, 21:37 authored by Hyatt, AD, Boyle, DG, Olsen, V, Boyle, DB, Berger, L, Obendorf, D, Dalton, AS, Kriger, K, Hero, M, Hines, H, Phillott, R, Campbell, R, Marantelli, G, Gleason, F, Colling, A
Batrachochytrium dendrobatidis is a fungus belonging to the Phylum Chytridiomycota, Class Chytridiomycetes, Order Chytridiales, and is the highly infectious aetiological agent responsible for a potentially fatal disease, chytridiomycosis, which is currently decimating many of the world's amphibian populations. The fungus infects 2 amphibian orders (Anura and Caudata), 14 families and at least 200 species and is responsible for at least 1 species extinction. Whilst the origin of the agent and routes of transmission are being debated, it has been recognised that successful management of the disease will require effective sampling regimes and detection assays. We have developed a range of unique sampling protocols together with diagnostic assays for the detection of B. dendrobatidis in both living and deceased tadpoles and adults. Here, we formally present our data and discuss them in respect to assay sensitivity, specificity, repeatability and reproducibility. We suggest that compliance with the recommended protocols will avoid the generation of spurious results, thereby providing the international scientific and regulatory community with a set of validated procedures which will assist in the successful management of chytridiomycosis in the future. © Inter-Research 2007.

History

Publication title

Diseases of Aquatic Organisms

Volume

73

Pagination

175-192

ISSN

0177-5103

Department/School

School of Natural Sciences

Publisher

Inter-Research

Place of publication

Germany

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the environmental sciences

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