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Diagnostic assays and sampling protocols for the detection of Batrachochytrium dendrobatidis

Citation

Hyatt, AD and Boyle, DG and Olsen, V and Boyle, DB and Berger, L and Obendorf, D and Dalton, AS and Kriger, K and Hero, M and Hines, H and Phillott, R and Campbell, R and Marantelli, G and Gleason, F and Colling, A, Diagnostic assays and sampling protocols for the detection of Batrachochytrium dendrobatidis, Diseases of Aquatic Organisms , 73, (3) pp. 175-192. ISSN 0177-5103 (2007) [Refereed Article]

DOI: doi:10.3354/dao073175

Abstract

Batrachochytrium dendrobatidis is a fungus belonging to the Phylum Chytridiomycota, Class Chytridiomycetes, Order Chytridiales, and is the highly infectious aetiological agent responsible for a potentially fatal disease, chytridiomycosis, which is currently decimating many of the world's amphibian populations. The fungus infects 2 amphibian orders (Anura and Caudata), 14 families and at least 200 species and is responsible for at least 1 species extinction. Whilst the origin of the agent and routes of transmission are being debated, it has been recognised that successful management of the disease will require effective sampling regimes and detection assays. We have developed a range of unique sampling protocols together with diagnostic assays for the detection of B. dendrobatidis in both living and deceased tadpoles and adults. Here, we formally present our data and discuss them in respect to assay sensitivity, specificity, repeatability and reproducibility. We suggest that compliance with the recommended protocols will avoid the generation of spurious results, thereby providing the international scientific and regulatory community with a set of validated procedures which will assist in the successful management of chytridiomycosis in the future. © Inter-Research 2007.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Zoology
Research Field:Zoology not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Dalton, AS (Mr Alexander Dalton)
ID Code:51666
Year Published:2007
Web of Science® Times Cited:343
Deposited By:Zoology
Deposited On:2007-08-01
Last Modified:2011-10-03
Downloads:0

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