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Detecting prey from DNA in predator scats: A comparison with morphological analysis, using Arctocephalus seals fed a known diet

Citation

Casper, RM and Jarman, SN and Deagle, BE and Gales, NJ and Hindell, MA, Detecting prey from DNA in predator scats: A comparison with morphological analysis, using Arctocephalus seals fed a known diet, Journal of Experimental Marine Biology and Ecology, 347, (1-2) pp. 144-154. ISSN 0022-0981 (2007) [Refereed Article]

DOI: doi:10.1016/j.jembe.2007.04.002

Abstract

The diet of free-living pinnipeds is most frequently estimated through identification of otoliths, squid mouth-parts and exoskeletons of prey in scats. This is because, although important prey types may not always be detected, sample collection is non-invasive and analysis is easy. Identification of prey DNA in scats is a nascent approach to determining the diet of marine vertebrates that may overcome some of the limitations of hard part analysis. This is the first study to experimentally compare the utility of genetic scatology for identifying consumption of prey types by seals with the occurrence of morphological remains of prey in scats. The occurrences of DNA and hard part remains of one squid and two fish taxa in scats of captive Arctocephalus seals fed mixed prey diets were compared. Both methods detected ingestion of these taxa 7.5-39.5 h prior to defaecation. Although all test prey had robust hard parts, detecting consumption during this period was 1.4 to 5.8 times more likely using genetic analysis than morphological analysis of scats. Based on frequency of occurrence calculations, neither method provided quantitative descriptions of the known diet. Identification of prey using DNA was not compromised by complexity of the diet; each test taxon was unambiguously detected against a background of a multi-species diet. Our results suggest that where diagnostic hard remains of prey are not well represented in scats, or the sample size is small, genetic scatology provides a valuable addition to morphological scat analysis for identifying the recent diet of free-living seals. © 2007 Elsevier B.V. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Environmental Sciences
Research Group:Environmental Science and Management
Research Field:Wildlife and Habitat Management
Objective Division:Environment
Objective Group:Ecosystem Assessment and Management
Objective Field:Ecosystem Assessment and Management of Antarctic and Sub-Antarctic Environments
Author:Casper, RM (Dr Ruth Casper)
Author:Deagle, BE (Dr Bruce Deagle)
Author:Hindell, MA (Professor Mark Hindell)
ID Code:50766
Year Published:2007
Web of Science® Times Cited:32
Deposited By:Zoology
Deposited On:2007-08-01
Last Modified:2011-11-04
Downloads:0

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