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DNA probes, targeting large sub-unit rRNA, for the rapid identification of the paralytic shellfish poison producing dinoflagellate, Gymnodinium catenatum

Citation

Rhodes, L and Smith, K and de Salas, MF, DNA probes, targeting large sub-unit rRNA, for the rapid identification of the paralytic shellfish poison producing dinoflagellate, Gymnodinium catenatum, New Zealand Journal of Marine and Freshwater Research, 41, (4) pp. 385-390. ISSN 0028-8330 (2007) [Refereed Article]

DOI: doi:10.1080/00288330709509928

Abstract

The dinoflagellate Gymnodinium catenatum was first observed in New Zealand at Manukau Harbour on the west coast of the North Island in May 2000. At that time, a strong correlation was evident between the micro-algal bloom and the occurrence of paralytic shellfish toxins (PSP) in shellfish. This paper describes the design and testing of oligonucletide probes targeting the large sub-unit (LSU) ribosomal RNA (rRNA) of G. catenatum. The probes were developed in fluorescent in situ hybridisation (FISH) and sandwich hybridisation assay (SHA) format to rapidly differentiate the target PSP producer from non-toxic look-alike dinoflagellates. Specificity was affirmed by testing the probes against dinoflagellate and flagellate isolates. © The Royal Society of New Zealand 2007.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Fish Pests and Diseases
Objective Division:Environment
Objective Group:Control of Pests, Diseases and Exotic Species
Objective Field:Control of Pests, Diseases and Exotic Species in Marine Environments
Author:de Salas, MF (Dr Miguel de Salas)
ID Code:50587
Year Published:2007
Web of Science® Times Cited:8
Deposited By:Plant Science
Deposited On:2007-08-01
Last Modified:2008-05-26
Downloads:0

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