eCite Digital Repository

Ultrastructural and biochemical analysis of a new mutation in Chlamydomonas reinhardtii affecting the central pair apparatus

Citation

Vucica, Y and Diener, DR and Rosenbaum, JL and Koutoulis, A, Ultrastructural and biochemical analysis of a new mutation in Chlamydomonas reinhardtii affecting the central pair apparatus, Protoplasma, 232, (1-2) pp. 121-130. ISSN 0033-183X (2007) [Refereed Article]

DOI: doi:10.1007/s00709-007-0273-8

Abstract

We present a new Chlamydomonas reinhardtii flagellar mutant in which central pair projections are missing and the central pair microtubules are twisted along the length of the flagellum. We have named this mutant tcp1 for twisted central pair. Immunoblots using an antibody that recognizes the heavy chain of sea urchin kinesin reveal that a 70 kDa protein present in wild-type and pf18 (central pairless) axonemes is absent in tcp1, suggesting the presence of an uncharacterized kinesin associated with the central pair apparatus. We demonstrate that the kinesin-like protein Klp1 is not attached to central pair microtubules in tcp1, but rather is located in, or is part of, a region we have termed the internal axonemal matrix. It is proposed that this matrix acts as a scaffold for axonemal proteins that may also be associated with the central pair apparatus. © 2007 Springer-Verlag.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Biochemistry and Cell Biology not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Vucica, Y (Ms Yvonne Vucica)
Author:Koutoulis, A (Professor Anthony Koutoulis)
ID Code:50561
Year Published:2007
Deposited By:Plant Science
Deposited On:2007-08-01
Last Modified:2008-05-26
Downloads:0

Repository Staff Only: item control page