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Proteomics identifies enhanced expression of stefin A in neonatal murine skin compared with adults: functional implications

Citation

Scott, DK and Lord, R and Muller, HK and Malley, RC and Woods, GM, Proteomics identifies enhanced expression of stefin A in neonatal murine skin compared with adults: functional implications, British Journal of Dermatology, 156, (6) pp. 1156-1162. ISSN 0007-0963 (2007) [Refereed Article]

DOI: doi:10.1111/j.1365-2133.2007.07875.x

Abstract

Background: Skin develops through a process of epidermal proliferation, maturation, and remodelling of the epidermis and dermis. This period also involves the maturation of the skin immune system, such that antigen applied though the skin of a neonatal mouse always results in immunosuppression, whereas in adults, immunity will occur. Objectives: Using proteomics, to identify proteins uniquely involved in the development of the skin and skin immune system. Methods: Proteins were extracted from whole skin of mice aged 4 and 21 days, and separated using two-dimensional electrophoresis. Results: Of the 25 proteins that were sequenced by peptide mass fingerprinting with matrix-assisted laser desorption/ionization-time of flight-mass spectrometry, three were known markers of keratinocyte differentiation and proliferation. These were cyclophilin A, epidermal fatty acid binding protein 5 and stefin A. Of interest were the two isoforms of stefin A, an intracellular protease inhibitor, found in neonatal skin. The strong expression of stefin A in neonates was confirmed by immunohistochemical analysis, suggesting an important role in the development of the epidermis. Additionally, Western blotting identified two larger isoforms in adult skin, revealing a change in the stefin A during development. Conclusions: We propose that stefin A is involved in development of the skin, that development of the skin and of immune function is linked, and that stefin A has an important function in neonatal skin and potentially the neonatal immune response. © 2007 The Authors.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Cellular Immunology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Immune System and Allergy
Author:Scott, DK (Ms Deborah Scott)
Author:Lord, R (Dr Roger Lord)
Author:Muller, HK (Professor Konrad Muller)
Author:Malley, RC (Dr Roslyn Malley)
Author:Woods, GM (Professor Gregory Woods)
ID Code:50287
Year Published:2007
Web of Science® Times Cited:15
Deposited By:Menzies Institute for Medical Research
Deposited On:2007-08-01
Last Modified:2012-12-12
Downloads:0

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