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Improved Prediction of Malt Fermentability by Measurement of the Diastatic Power Enzymes, β-Amylase, α-Amylase, and Limit Dextrinase: II. Impact of Barley Genetics, Growing Environment and Gibberellin on Levels of α-Amylase and Limit Dextinase in Malt

Citation

Evans, DE and Li, C and Harasymow, S and Roumeliotis, S and Eglinton, JK, Improved Prediction of Malt Fermentability by Measurement of the Diastatic Power Enzymes, β-Amylase, α-Amylase, and Limit Dextrinase: II. Impact of Barley Genetics, Growing Environment and Gibberellin on Levels of α-Amylase and Limit Dextinase in Malt, Journal American Society of Brewing Chemists, 67, (1) pp. 14-22. ISSN 0361-0470 (2009) [Refereed Article]

DOI: doi:10.1094/ASBCJ-2008-1206-01

Abstract

The determination of the levels of the diastatic power enzymes (DPEs) β-amylase, limit dextrinase, and α-amylase has previously been shown to predict barley malt fermentability. Using micromalted samples of barley from different genotypes and growing environments, it was demonstrated that both these factors were important in determining the level of DPEs in malt. In terms of genotypic effects, a trial of breeders' lines and varieties showed substantial variation in the levels of total β-amylase (means 455-914 U/g), total limit dextrinase (means 268-603 U/kg), and α-amylase (means 154-316 U/g). The application of gibberellin (GA) during malting resulted in substantial increases in the levels of total limit dextrinase and α-amylase and the extent of modification (KI). However, the level of total β-amylase was relatively unchanged. It was also observed that the levels of total limit dextrinase and α-amylase and extent of KI generally were highly correlated (r » 0.6-0.7), which was attributed to the sensitivity of each of these malt quality factors to GA. The results are discussed in terms of their practical importance to barley breeders and maltsters seeking to supply malt that satisfies the malt fermentability requirements of their brewing customers.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Crop and Pasture Biochemistry and Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Winter Grains and Oilseeds
Objective Field:Barley
Author:Evans, DE (Dr David Evans)
ID Code:50228
Year Published:2009
Web of Science® Times Cited:12
Deposited By:Agricultural Science
Deposited On:2008-07-22
Last Modified:2013-01-31
Downloads:0

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