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Differential gene expression of Listeria monocytogenes during high hydrostatic pressure processing

Citation

Bowman, JP and Bittencourt, C and Ross, T, Differential gene expression of Listeria monocytogenes during high hydrostatic pressure processing, Microbiology, 154, (2) pp. 462-475. ISSN 1350-0872 (2008) [Refereed Article]

DOI: doi:10.1099/mic.0.2007/010314-0

Abstract

High hydrostatic pressure processing (HPP) is currently being used as a treatment for certain foods to control the presence of food-borne pathogens, such as Listeria monocytogenes. Genomic microarray analysis was performed to determine the effects of HPP on L. monocytogenes in order to understand how it responds to mechanical stress injury. Reverse transcriptase PCR analysis of tufA and rpoC indicated that the reduction of mRNA expression in HPP-treated cells was dependent on intensity and time of the treatment. Treatments of 400 and 600 MPa for 5 min on cells in the exponential growth phase, though leading to partial or complete cellular inactivation, still resulted in measurable relative differential gene expression. Gene set enrichment analysis indicated that HPP induced increased expression of genes associated with DNA repair mechanisms, transcription and translation protein complexes, the septal ring, the general protein translocase system, flagella assemblage and chemotaxis, and lipid and peptidoglycan biosynthetic pathways. On the other hand, HPP appears to suppress a wide range of energy production and conversion, carbohydrate metabolism and virulence-associated genes accompanied by strong suppression of the SigB and PrfA regulons. HPP also affected genes controlled by the pleotrophic regulator CodY. HPP-induced cellular damage appears to lead to increased expression of genes linked to sections of the cell previously shown in bacteria to be damaged or altered during HPP exposure and suppression of gene expression associated with cellular growth processes and virulence. © 2008 SGM.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Bacteriology
Objective Division:Health
Objective Group:Public Health (excl. Specific Population Health)
Objective Field:Food Safety
Author:Bowman, JP (Associate Professor John Bowman)
Author:Bittencourt, C (Mr Claudio Bittencourt)
Author:Ross, T (Associate Professor Tom Ross)
ID Code:50221
Year Published:2008
Web of Science® Times Cited:43
Deposited By:Agricultural Science
Deposited On:2008-04-08
Last Modified:2012-03-05
Downloads:0

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