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A randomized controlled clinical trial to determine the optimum duration of G-CSF priming prior to BM stem cell harvesting
Citation
Lowenthal, RM and Ragg, SJ and Anderson, J and Nicholson, L and Harrup, R and Tuck, DM, A randomized controlled clinical trial to determine the optimum duration of G-CSF priming prior to BM stem cell harvesting, Cytotherapy, 9, (2) pp. 158-164. ISSN 1465-3249 (2007) [Refereed Article]
DOI: doi:10.1080/14653240601182820
Abstract
Background: Harvesting of hemopoietic stem cells (HSC) from G-CSF-primed BM for autologous transplantation is an alternative to collection of unprimed BM or G-CSF-primed peripheral blood (PB). However, the optimum number of days of G-CSF administration for this purpose is unknown. We set out to determine whether cell yields could be optimized by varying the number of days of G-CSF administration prior to BM stem cell harvesting. Methods: We conducted a randomized controlled single-centertrial of 6 days (the standard) vs. 4 days of G-CSF administration and compared yields of total nucleated cells (TNC), CD34+ HSC and CFU-GM cells per kilogram patient body weight. Statistical analysis was by Student's t-test. Results: Twenty-four patients were enrolled; 13 received 6 days and 11 received 4 days of G-CSF administration. Analysis of the first harvest aspirate showed higher proportions of CD34+ HSC (P = 0.02) and CFU-GM (P = 0.03) in the 4-day group. For the 6-day and 4-day groups, respectively, the median yield of TNC/kg was 6.5 × 108 and 5.4 × 108 (P = 0.28), of CD34+ cells/kg 0.56 ×106 and 0.98 × 106 (P = 0.04) and of CFU-GM cells/kg 1.66 × 105 and 1.55 × 105 (P = 0.75). Discussion: These results suggest that by 6 days the HSC-stimulating effect of G-CSF has passed its peak and that 4 days should be adopted as the standard for G-CSF priming prior to BM stem cell harvesting for autologous transplantation.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Biomedical and Clinical Sciences |
| Research Group: | Oncology and carcinogenesis |
| Research Field: | Oncology and carcinogenesis not elsewhere classified |
| Objective Division: | Health |
| Objective Group: | Clinical health |
| Objective Field: | Clinical health not elsewhere classified |
| UTAS Author: | Lowenthal, RM (Professor Ray Lowenthal) |
| UTAS Author: | Ragg, SJ (Dr Scott Ragg) |
| UTAS Author: | Harrup, R (Dr Rosemary Harrup) |
| UTAS Author: | Tuck, DM (Mrs Deirdre Tuck) |
| ID Code: | 50184 |
| Year Published: | 2007 |
| Web of Science® Times Cited: | 4 |
| Deposited By: | Medicine |
| Deposited On: | 2007-08-01 |
| Last Modified: | 2009-09-17 |
| Downloads: | 0 |
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