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Identification and expression of natriuretic peptide receptor type-A and -B mRNA in freshwater and seawater rainbow trout

Citation

Nankervis, S and Powell, MD and McLeod, J and Toop, T, Identification and expression of natriuretic peptide receptor type-A and -B mRNA in freshwater and seawater rainbow trout, Journal of Comparative Physiology B: biochemical, systemic, and environmental physiology, 177, (2) pp. 259-267. ISSN 0174-1578 (2007) [Refereed Article]

DOI: doi:10.1007/s00360-006-0127-2

Abstract

Natriuretic peptide receptors mediate the physiological response of natriuretic peptide hormones. One of the natriuretic peptide receptor types is the particulate guanylyl cyclase receptors, of which there are two identified: NPR-A and NPR-B. In fishes, these have been sequenced and characterized in eels, medaka, and dogfish shark (NPR-B only). The euryhaline rainbow trout provides an opportunity to further pursue examination of the system in teleosts. In this study, partial rainbow trout NPR-A-like and NPR-B-like mRNA sequences were identified via PCR and cloning. The sequence information was used in real-time PCR to examine mRNA expression in a variety of tissues of freshwater rainbow trout and rainbow trout acclimated to 35 parts per thousand seawater for a period of 10 days. In the excretory kidney and posterior intestine, real-time PCR analysis showed greater expression of NPR-B in freshwater fish than in those adapted to seawater; otherwise, there was no difference in the expression of the individual receptors in fresh water or seawater. In general, the expression of the NPR-A and NPR-B type receptors was quite low. These findings indicate that NPR-A and NPR-B mRNA expression is minimally altered under the experimental regime used in this study. © 2006 Springer-Verlag.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Powell, MD (Dr Mark Powell)
ID Code:50046
Year Published:2007
Web of Science® Times Cited:3
Deposited By:TAFI - Aquaculture
Deposited On:2007-08-01
Last Modified:2011-11-04
Downloads:0

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