Identification and expression of natriuretic peptide receptor type-A and -B mRNA in freshwater and seawater rainbow trout
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Nankervis, S and Powell, MD and McLeod, J and Toop, T, Identification and expression of natriuretic peptide receptor type-A and -B mRNA in freshwater and seawater rainbow trout, Journal of Comparative Physiology B: biochemical, systemic, and environmental physiology, 177, (2) pp. 259-267. ISSN 0174-1578 (2007) [Refereed Article]
Natriuretic peptide receptors mediate the physiological response of natriuretic peptide hormones. One of the natriuretic peptide receptor types is the particulate guanylyl cyclase receptors, of which there are two identified: NPR-A and NPR-B. In fishes, these have been sequenced and characterized in eels, medaka, and dogfish shark (NPR-B only). The euryhaline rainbow trout provides an opportunity to further pursue examination of the system in teleosts. In this study, partial rainbow trout NPR-A-like and NPR-B-like mRNA sequences were identified via PCR and cloning. The sequence information was used in real-time PCR to examine mRNA expression in a variety of tissues of freshwater rainbow trout and rainbow trout acclimated to 35 parts per thousand seawater for a period of 10 days. In the excretory kidney and posterior intestine, real-time PCR analysis showed greater expression of NPR-B in freshwater fish than in those adapted to seawater; otherwise, there was no difference in the expression of the individual receptors in fresh water or seawater. In general, the expression of the NPR-A and NPR-B type receptors was quite low. These findings indicate that NPR-A and NPR-B mRNA expression is minimally altered under the experimental regime used in this study. © 2006 Springer-Verlag.
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