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Effect of cloned inhibitor resistant TEM B-lactamases on the susceptibility of H. influenzae to amoxicillin/clavulanate

Citation

Tristram, SG and Burdach, JG, Effect of cloned inhibitor resistant TEM B-lactamases on the susceptibility of H. influenzae to amoxicillin/clavulanate, Journal of Antimicrobial Chemotherapy , 60, (5) pp. 1151-1154 . ISSN 0305-7453 (2007) [Refereed Article]

DOI: doi:10.1093/jac/dkm311

Abstract

Objectives: To determine the effect of cloned inhibitor-resistant TEM β-lactamases (IRTs) on the susceptibility of Haemophilus influenzae to amoxicillin/clavulanate. Methods: IRT-2, -4 and -5 genes with various promoters were cloned into control strains of H. influenzae and the amoxicillin/clavulanate MICs were measured using Etests. Results: IRT enzymes were able to raise the amoxicillin/clavulanate MICs to between 0.38/0.19 and 4.0/2.0 mg/L depending on the IRT and promoter genotype, compared with MICs of 0.19/0.09 to 0.5/0.25 mg/L for the corresponding strains with TEM-1. Strains with an IRT and altered penicillin-binding proteins had amoxicillin/clavulanate MICs as high as 8.0/4.0 mg/L. Conclusions: Cloned IRT enzymes in H. influenzae raise the amoxicillin/ clavulanate MICs to an extent comparable to naturally occurring strains with decreased amoxicillin/clavulanate susceptibility. © The Author 2007. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Medical Microbiology
Research Field:Medical Bacteriology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Infectious Diseases
Author:Tristram, SG (Dr Stephen Tristram)
Author:Burdach, JG (Mr Jonathan Burdach)
ID Code:50023
Year Published:2007
Web of Science® Times Cited:4
Deposited By:Health Sciences A
Deposited On:2007-08-01
Last Modified:2009-10-20
Downloads:0

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