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Evaluation of quantitative polymerase chain reaction to assess nosZ gene prevalence in mixed microbial communities

Citation

Sicilliano, SD and Wai, M and Powell, S, Evaluation of quantitative polymerase chain reaction to assess nosZ gene prevalence in mixed microbial communities, Canadian Journal of Microbiology, 53, (5) pp. 636-642. ISSN 0008-4166 (2007) [Refereed Article]

DOI: doi:10.1139/W07-014

Abstract

The usefulness of quantitative polymerase chain reaction (QPCR) to measure nosZ gene prevalence in a multitemplate reaction was assessed by comparing 19 nosZ template DNA samples and 91 model communities. Efficiencies of the QPCR varied but were not significantly different among nosZ genotypes and were not linked to genetic distance from Ralstonia eutropha. nosZ genotype QPCR efficiencies obtained from isolated denitrifiers were higher (84.8%) than those obtained from excised denaturing gradient gel electrophoresis bands or clones of PCR products from total community DNA (ca. 60%). Analysis of the model communities indicated that QPCR accurately predicts gene prevalence in communities composed of up to six templates. © 2007 NRC.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbial Ecology
Objective Division:Environment
Objective Group:Ecosystem Assessment and Management
Objective Field:Ecosystem Assessment and Management of Marine Environments
Author:Powell, S (Dr Shane Powell)
ID Code:49917
Year Published:2007
Web of Science® Times Cited:2
Deposited By:Agricultural Science
Deposited On:2007-08-01
Last Modified:2009-11-05
Downloads:0

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