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Cyclopropane fatty acids improve Escherichia coli survival in acidified minimal media by reducing membrane permeability to H+ and enhanced ability to extrude H+

Citation

Shabala, Lana and Ross, T, Cyclopropane fatty acids improve Escherichia coli survival in acidified minimal media by reducing membrane permeability to H+ and enhanced ability to extrude H+, Research in Microbiology, 159, (6) pp. 458-461. ISSN 0923-2508 (2008) [Refereed Article]

DOI: doi:10.1016/j.resmic.2008.04.011

Abstract

The physiological role of cyclopropane fatty acids (CFAs) in acid stress resistance was studied by in situ microelectrode H+ flux measurements of Escherichia coli Frag1 and its isogenic CFA-deficient mutant JBM 1. After exposure to pH 4 for 16 h, net H+ influx in JBM 1 was twice that of the parent strain. H+ flux stabilisation at pH 6 after acid stress took more time in the cfa- mutant. The data suggest increased proton permeability and decreased ability to extrude H+ in the absence of CFA, and they support the hypothesis that CFAs protect E. coli in acidic environments by decreasing membrane permeability to H+. Crown Copyright © 2008.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbial Ecology
Objective Division:Health
Objective Group:Public Health (excl. Specific Population Health)
Objective Field:Food Safety
Author:Shabala, Lana (Dr Lana Shabala)
Author:Ross, T (Associate Professor Tom Ross)
ID Code:49545
Year Published:2008
Web of Science® Times Cited:37
Deposited By:Agricultural Science
Deposited On:2008-08-01
Last Modified:2015-02-02
Downloads:0

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