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Cartesian-diver microrespirometry: a technique for the accurate measurement of respiratory flux in plant cells


Owen, JH and Laybourn-Parry, J, Cartesian-diver microrespirometry: a technique for the accurate measurement of respiratory flux in plant cells, Plant, Cell and Environment, 12, (3) pp. 323-330. ISSN 0140-7791 (1989) [Refereed Article]

DOI: doi:10.1111/j.1365-3040.1989.tb01947.x


Abstract. A Cartesian‐diver microrespirometer system is described which can be used to measure respiratory fluxes of oxygen accurately for cells of higher plants in a liquid phase. This microrespirometry technique has been adapted from protozoological and microfaunal studies to plant physiology. The Cartesian‐diver has considerable scope for investigation of oxygen flux in plant cells and has several advantages compared to the oxygen electrode in terms of sensitivity to changing oxygen levels in respiring material. Because the volumes of liquid are small in the Cartesian‐divers, diffusional distances arc measured in micrometres and there is no need for stirring to overcome diffusional problems, thus minimizing the risk of mechanical damage to the experimental material. In addition, only very small quantities of experimental material are required for the Cartesian‐diver which is invaluable where only limited amounts of tissue or numbers of cells can be obtained. Examples of respiratory oxygen consumption by protoplasts from intercalary meristematic regions of light‐grown barley (Hordeum vulgare L.c.v. Patty) seedlings, in response to abscisic and gibberellic acids, are presented. The advantages and disadvantages of Cartesian‐diver microrespirometry compared to oxygen electrodes are also discussed. Copyright © 1989, Wiley Blackwell. All rights reserved

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Ecology
Research Field:Marine and estuarine ecology (incl. marine ichthyology)
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the environmental sciences
UTAS Author:Laybourn-Parry, J (Professor Johanna Laybourn-Parry)
ID Code:49501
Year Published:1989
Web of Science® Times Cited:2
Deposited By:Research Division
Deposited On:2007-12-15
Last Modified:2011-08-30

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