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Ultrasonic imaging of biofilms utilizing echoes from the biofilm/air interface


Holmes, AK and Laybourn-Parry, J and Parry, JD and Unwin, ME and Challis, RE, Ultrasonic imaging of biofilms utilizing echoes from the biofilm/air interface, IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control, 53, (1) pp. 185-192. ISSN 0885-3010 (2006) [Refereed Article]

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Copyright © 2006 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.

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DOI: doi:10.1109/TUFFC.2006.1588404


Ultrasonic imaging of biofilms in water is difficult due to the very low contrast in acoustic impedance between the biofilm and water. In this paper, biofilms exposed to moist air are scanned through the substrate in order to obtain echoes from the biofilm/air interface. A 50 MHz scanning system was used to scan 1 mm x 1 mm areas of biofilms in a 10 um grid pattern. Two fast Fourier transform (FFT) based methods for enhancement of the film thickness measurement resolution are compared. Using these techniques, the surface topography of biofilms with thickness less than the acoustic wavelength can be imaged.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbial ecology
Objective Division:Environmental Management
Objective Group:Management of Antarctic and Southern Ocean environments
Objective Field:Biodiversity in Antarctic and Southern Ocean environments
UTAS Author:Laybourn-Parry, J (Professor Johanna Laybourn-Parry)
ID Code:49093
Year Published:2006
Web of Science® Times Cited:5
Deposited By:Research Division
Deposited On:2007-11-13
Last Modified:2011-02-22
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