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The use of ultrasonic imaging to evaluate the effect of protozoan grazing and movement on the topography of bacterial biofilms


Parry, JD and Holmes, AK and Unwin, ME and Laybourn-Parry, J, The use of ultrasonic imaging to evaluate the effect of protozoan grazing and movement on the topography of bacterial biofilms, Letters in Applied Microbiology, 45, (4) pp. 364-370. ISSN 0266-8254 (2007) [Refereed Article]

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DOI: doi:10.1111/j.1472-765X.2007.02213.x


Aims: This study evaluated the effect of protozoan movement and grazing on the topography of a dual-bacterial biofilm using both conventional light microscopy and a new ultrasonic technique. Methods and Results: Coupons of dialysis membrane were incubated in Chalkley’s medium for 3 days at 23!C in the presence of bacteria (Pseudomonas aeruginosa and Klebsiella aerogenes) alone, or in co-culture with the flagellate Bodo designis, the ciliate Tetrahymena pyriformis or the amoeba Acanthamoeba castellanii. Amoebic presence resulted in a confluent biofilm similar to the bacteria-only biofilm while the flagellate and ciliate created more diverse biofilm topographies comprising bacterial microcolonies and cavities. Conclusions: The four distinct biofilm topographies were successfully discerned with ultrasonic imaging and the method yielded information similar to that obtained with conventional light microscopy. Significance and Impact of the Study: Ultrasonic imaging provides a potential way forward in the development of a portable, nondestructive technique for profiling the topography of biofilms in situ, which might aid in the future management of biofouling.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbial ecology
Objective Division:Environmental Management
Objective Group:Management of Antarctic and Southern Ocean environments
Objective Field:Biodiversity in Antarctic and Southern Ocean environments
UTAS Author:Laybourn-Parry, J (Professor Johanna Laybourn-Parry)
ID Code:49020
Year Published:2007
Web of Science® Times Cited:10
Deposited By:Research Division
Deposited On:2007-11-12
Last Modified:2009-06-09

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