eCite Digital Repository

Analysis of the role of pglI in pilin glycosylation of Neisseria meningitidis


Warren, MJ and Roddam, LF and Power, PM and Terry, TD and Jennings, MP, Analysis of the role of pglI in pilin glycosylation of Neisseria meningitidis, FEMS Immunology and Medical Microbiology, 41, (1) pp. 43-50. ISSN 0928-8244 (2004) [Refereed Article]

DOI: doi:10.1016/j.femsim.2004.01.002


Pilin is the major subunit of the essential virulence factor pili and is glycosylated at Ser63. In this study we investigated the gene pglI to determine whether it is involved in the biosynthesis of the pilin-linked glycan of Neisseria meningitidis strain C311#3. A N. meningitidis C311#3pglI mutant resulted in a change of apparent molecular weight in SDS-PAGE and altered binding of antisera, consistent with a role in the biosynthesis of the pilin-linked glycan. These data, in conjunction with homology with well-characterised acyltransferases suggests a specific role for pglI in the biosynthesis of the basal 2,4-diacetamido-2,4,6-trideoxyhexose residue of the pilin-linked glycan. © 2004 Published by Elsevier B.V. on behalf of the Federation of European Microbiological Societies.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Medical biochemistry and metabolomics
Research Field:Medical biochemistry and metabolomics not elsewhere classified
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Roddam, LF (Dr Louise Roddam)
ID Code:48870
Year Published:2004
Web of Science® Times Cited:29
Deposited By:Menzies Institute for Medical Research
Deposited On:2007-11-05
Last Modified:2011-10-04

Repository Staff Only: item control page