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Compatible solutes reduce ROS-induced potassium efflux in Arabidopsis roots

Citation

Cuin, TA and Shabala, SN, Compatible solutes reduce ROS-induced potassium efflux in Arabidopsis roots, Plant Cell and Environment, 30, (7) pp. 875-885. ISSN 0140-7791 (2007) [Refereed Article]

DOI: doi:10.1111/j.1365-3040.2007.01674.x

Abstract

Reactive oxygen species (ROS) are known to be primarily responsible for the impairment of cellular function under numerous abiotic and biotic stress conditions. In this paper, using non-invasive microelectrode ion flux measuring (MIFE) system, we show that the application of a hydroxyl radical (OH •)-generating Cu 2+/ascorbate (Cu/a) mixture to Arabidopsis roots results in a massive, dose-dependent efflux of K + from epidermal cells in the elongation zone. Pharmacological experiments suggest that both outward-rectifying K + channels and non-selective cation channels (NSCCs) mediate such effluxes. Low (5 mM) concentrations of compatible solutes (glycine betaine, proline, mannitol, trehalose or myo-inositol) significantly reduces OH •-induced K + efflux, similar to our previous reports for NaCl-induced K + efflux. Importantly, a significant reduction in K + efflux was found using osmolytes with no reported free radical scavenging activity, as well as those for which a role in free radical scavenging has been demonstrated. This indicates that compatible solutes must play other (regulatory) roles, in addition to free radical scavenging, in mitigating the damaging effects of oxidative stress. © 2007 The Authors.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Cuin, TA (Dr Tracey Cuin)
Author:Shabala, SN (Professor Sergey Shabala)
ID Code:48795
Year Published:2007
Web of Science® Times Cited:142
Deposited By:Agricultural Science
Deposited On:2007-08-01
Last Modified:2009-09-22
Downloads:0

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