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Alpha-synuclein is upregulated in neurones in response to chronic oxidative stress and is associated with neuroprotection

journal contribution
posted on 2023-05-16, 20:22 authored by Quilty, MC, Anna KingAnna King, Gai, WP, Pountney, DL, Adrian WestAdrian West, James VickersJames Vickers, Tracey DicksonTracey Dickson
Chronic oxidative stress has been linked to the neurodegenerative changes characteristic of Parkinson's disease, particularly alpha-synuclein accumulation and aggregation. However, it remains contentious whether these alpha-synuclein changes are cytotoxic or neuroprotective. The current study utilised long-term primary neural culture techniques with antioxidant free media to study the cellular response to chronic oxidative stress. Cells maintained in antioxidant free media were exquisitely more vulnerable to acute exposure to hydrogen peroxide, yet exposure of up to 10 days in antioxidant free media did not lead to morphological alterations in neurones or glia. However, a subpopulation of neurones demonstrated a significant increase in the level of alpha-synuclein expressed within the cell body and at synaptic sites. This subset of neurones was also more resistant to apoptotic changes following exposure to antioxidant free media relative to other neurones. These data indicate that increased alpha-synuclein content is associated with neuroprotection from relatively low levels of oxidative stress.

History

Publication title

Experimental Neurology

Volume

199

Pagination

249-256

ISSN

0014-4886

Department/School

School of Psychological Sciences

Publisher

Academic Press

Place of publication

USA

Repository Status

  • Restricted

Socio-economic Objectives

Clinical health not elsewhere classified

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    University Of Tasmania

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