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Neoparamoeba perurans n. sp., an agent of amoebic gill disease of Atlantic Salmon (Salmo salar)

Citation

Young, ND and Crosbie, PBB and Adams, MB and Nowak, BF and Morrison, RN, Neoparamoeba perurans n. sp., an agent of amoebic gill disease of Atlantic Salmon (Salmo salar), International Journal for Parasitology, 37, (13) pp. 1469-1481. ISSN 0020-7519 (2007) [Refereed Article]

DOI: doi:10.1016/j.ijpara.2007.04.018

Abstract

Amoebic gill disease (AGD) is a potentially fatal disease of some marine fish. Two amphizoic amoebae Neoparamoeba pemaquidensis and Neoparamoeba branchiphila have been cultured from AGD-affected fish, yet it is not known if one or both are aetiological agents. Here, we PCR amplified the 18S rRNA gene of non-cultured, gill-derived (NCGD) amoebae from AGD-affected Atlantic salmon (Salmo salar) using N. pemaquidensis and N. branchiphila-specific oligonucleotides. Variability in PCR amplification led to comparisons of 18S rRNA and 28S rRNA gene sequences from NCGD and clonal cultured, gill-derived (CCGD) N. pemaquidensis and N. branchiphila. Phylogenetic analyses inferred from either 18S or 28S rRNA gene sequences unambiguously segregated a lineage consisting of NCGD amoebae from other members of the genus Neoparamoeba. Species-specific oligonucleotide probes that hybridise 18S rRNA were designed, validated and used to probe gill tissue from AGD-affected Atlantic salmon. The NCGD amoebae-specific probe bound AGD-associated amoebae while neither N. pemaquidensis nor N. branchiphila were associated with AGD-lesions. Together, these data indicate that NCGD amoebae are a new species, designated Neoparamoeba perurans n.sp. and this is the predominant aetiological agent of AGD of Atlantic salmon cultured in Tasmania, Australia. © 2007 Australian Society for Parasitology Inc.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Young, ND (Mr Neil Young)
Author:Crosbie, PBB (Dr Philip Crosbie)
Author:Adams, MB (Dr Mark Adams)
Author:Nowak, BF (Professor Barbara Nowak)
Author:Morrison, RN (Dr Richard Morrison)
ID Code:48400
Year Published:2007
Web of Science® Times Cited:83
Deposited By:TAFI - Aquaculture
Deposited On:2007-08-01
Last Modified:2009-07-09
Downloads:0

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