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Neoparamoeba perurans n. sp., an agent of amoebic gill disease of Atlantic Salmon (Salmo salar)


Young, ND and Crosbie, PBB and Adams, MB and Nowak, BF and Morrison, RN, Neoparamoeba perurans n. sp., an agent of amoebic gill disease of Atlantic Salmon (Salmo salar), International Journal for Parasitology, 37, (13) pp. 1469-1481. ISSN 0020-7519 (2007) [Refereed Article]

DOI: doi:10.1016/j.ijpara.2007.04.018


Amoebic gill disease (AGD) is a potentially fatal disease of some marine fish. Two amphizoic amoebae Neoparamoeba pemaquidensis and Neoparamoeba branchiphila have been cultured from AGD-affected fish, yet it is not known if one or both are aetiological agents. Here, we PCR amplified the 18S rRNA gene of non-cultured, gill-derived (NCGD) amoebae from AGD-affected Atlantic salmon (Salmo salar) using N. pemaquidensis and N. branchiphila-specific oligonucleotides. Variability in PCR amplification led to comparisons of 18S rRNA and 28S rRNA gene sequences from NCGD and clonal cultured, gill-derived (CCGD) N. pemaquidensis and N. branchiphila. Phylogenetic analyses inferred from either 18S or 28S rRNA gene sequences unambiguously segregated a lineage consisting of NCGD amoebae from other members of the genus Neoparamoeba. Species-specific oligonucleotide probes that hybridise 18S rRNA were designed, validated and used to probe gill tissue from AGD-affected Atlantic salmon. The NCGD amoebae-specific probe bound AGD-associated amoebae while neither N. pemaquidensis nor N. branchiphila were associated with AGD-lesions. Together, these data indicate that NCGD amoebae are a new species, designated Neoparamoeba perurans n.sp. and this is the predominant aetiological agent of AGD of Atlantic salmon cultured in Tasmania, Australia.

Item Details

Item Type:Refereed Article
Keywords:amoebic gill disease, Neoparamoeba pemaquidensis, Neoparamoeba branchiphila, Neoparamoeba perurans, Salmo salar, in situ hybridisation, rRNA, 18S, 28S
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Fisheries - aquaculture not elsewhere classified
UTAS Author:Young, ND (Mr Neil Young)
UTAS Author:Crosbie, PBB (Dr Philip Crosbie)
UTAS Author:Adams, MB (Dr Mark Adams)
UTAS Author:Nowak, BF (Professor Barbara Nowak)
UTAS Author:Morrison, RN (Dr Richard Morrison)
ID Code:48400
Year Published:2007
Web of Science® Times Cited:135
Deposited By:TAFI - Aquaculture
Deposited On:2007-08-01
Last Modified:2021-03-19

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