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Non-lethal imaging techniques for crab spermathecae

journal contribution
posted on 2023-05-16, 20:12 authored by Caleb GardnerCaleb Gardner, Rush, M, Bevilacqua, T
Techniques for collecting information on spermathecae without dissection were evaluated on the giant crab Pseudocarcinus gigas. Techniques tested were: biopsy, ultrasound, conventional x-radiography, computerized tomography (CT) scans, and magnetic resonance imaging (MRI). Attempts at biopsy and ultrasound imaging were unsuccessful. Spermathecae were imaged by x-radiography, although resolution was poor, suggesting that it can be applied only to gain general information, such as determining whether mating has occurred. High resolution images were produced with CT and MRI. Resolution by MRI is of such detail that internal structure of spermathecae is imaged. Nonlethal techniques allow animals to be used repeatedly, which permits monitoring of changes during mating, sperm storage, and extrusion.

History

Publication title

Journal of Crustacean Biology

Volume

18

Pagination

64-69

ISSN

0278-0372

Department/School

Institute for Marine and Antarctic Studies

Publisher

Crustacean Society

Place of publication

United States

Repository Status

  • Restricted

Socio-economic Objectives

Fisheries - aquaculture not elsewhere classified

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