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Flow gradient liquid chromatography using a coated anion exchange microcolumn


Nesterenko, EP and Barron, LP and Nesterenko, PN and Paull, B, Flow gradient liquid chromatography using a coated anion exchange microcolumn, Journal of Separation Science, 29, (2) pp. 228-235. ISSN 1615-9306 (2006) [Refereed Article]

DOI: doi:10.1002/jssc.200500335


Ion chromatography on a 4.0-mm-long (3.0 min ID) ion exchange column is presented. Using a 10 mM phosphate buffer (pH 2.22) the separation of up to six UV-absorbing anions was obtained using the microcolumn, containing 5 μm RP support (Phenomenex Gemini) coated with the zwitterionic surfactant, (N-dodecyl-N,N-dimethyl-ammonio) undecanoate. The short analytical column facilitated the application of a flow gradient programme over the flow range 0.3-5 mL/min resulting in optimum resolution of nitrite, nitrate, benzoate, iodide, thiocyanate and trichloroacetate in less than 10 min. The effect of both eluent concentration and pH on the retention of six selected anions was investigated, showing a strong pH capacity dependence. The microcolumn was found to exhibit no selectivity towards chloride and so was well suited to the analysis of saline samples. To illustrate this, the rapid analysis of a concentrated iodized table salt sample (20 g/L) was carried out. Following standard addition, a concentration of 3.55 ± 0.05 μg iodide/g and 1.05 ± 0.02 μg iodate/g in the solid salt sample was determined. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Nesterenko, PN (Professor Pavel Nesterenko)
UTAS Author:Paull, B (Professor Brett Paull)
ID Code:47747
Year Published:2006
Web of Science® Times Cited:10
Deposited By:Chemistry
Deposited On:2007-09-20
Last Modified:2011-08-18

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