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Latex-functionalized monolithic columns for the separation of carbohydrates by micro anion-exchange chromatography

Citation

Hilder, EF and Svec, F and Frechet, JMJ, Latex-functionalized monolithic columns for the separation of carbohydrates by micro anion-exchange chromatography, Journal of Chromatography A, 1053, (1-2) pp. 101-106. ISSN 0021-9673 (2004) [Refereed Article]

DOI: doi:10.1016/j.chroma.2004.06.125

Abstract

A novel stationary phase for micro ion chromatography has been prepared by coating a porous poly(butyl methacrylate-co-ethylene dimethacrylate-co-2- acrylamido-2-methyl-1-propanesulfonic acid) monolith with quaternary amine-functionalized latex particles via simple electrostatic binding. This stationary phase enabled the separation of saccharides in the mobile phase with a high-pH value consisting of aqueous ammonia solution in anion-exchange mode using evaporative light scattering for detection. Effects of both porous properties of the monolithic stationary phase and chromatographic conditions on the separation ability were studied. Under optimized conditions, an efficient separation of seven saccharides was achieved in less than 10 min. The stationary phase also enables the separation of saccharides obtained by the enzymatic hydrolysis of corn starch. © 2004 Elsevier B.V. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Hilder, EF (Professor Emily Hilder)
Author:Svec, F (Dr Frantisek Svec)
ID Code:47715
Year Published:2004
Web of Science® Times Cited:90
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2007-08-01
Last Modified:2007-09-20
Downloads:0

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